人源环状RNA_ROBO1（hsa_circ_ROBO1）与微小RNA-324-3p（miR-324-3p）竞争并上调NME1，以促进鼻咽癌的肿瘤转移。
Hsa_circ_ROBO1 competes with miR-324-3p and upregulates NME1 to promote tumor metastasis in nasopharyngeal carcinoma.
作者信息
Liang FenFeng, Zhao Hai, Liu GengChun, Huang MeiLing, Peng ZhengJia
机构信息
Department of Otolaryngology and Head Surgery, Xiangtan Central Hospital, Xiangtan, 411100, Hunan, China.
Department of Radiotherapy, Xiangtan Central Hospital, No.120, Heping Road, Yuhu District, Xiangtan, 411100, Hunan, China.
出版信息
Discov Oncol. 2024 Sep 2;15(1):398. doi: 10.1007/s12672-024-01220-9.
BACKGROUND
This work aims to explore circ_ROBO1's function in nasopharyngeal carcinoma (NPC).
METHODS
circ_ROBO1 expression in NPC tissues and cell lines was measured. The regulation of circ_ROBO1 and/or miR-324-3p on the proliferation, migration, invasion, and apoptosis of NPC cells was investigated by functional experiments. The interplay between circ_ROBO1, miR-324-3p, and NME1 was explored. Tumor growth and metastasis were studied in mice.
RESULTS
circ_ROBO1 was overexpressed in NPC. Knockdown of circ_ROBO1 repressed proliferation, migration, and invasion and induced apoptosis of NPC cells. Loss of circ_ROBO1 reduced tumor growth and metastasis in mice. circ_ROBO1 competed with miR-324-3p to upregulate NME1. Lowering miR-324-3p expression impaired the effect of knockdown of circ_ROBO1on NPC cells.
CONCLUSION
Overexpressed circ_ROBO1 promotes NPC development by modifying the miR-324-3p/NME1 axis.
背景
本研究旨在探讨环状RNA_ROBO1（circ_ROBO1）在鼻咽癌（NPC）中的作用。
方法
检测circ_ROBO1在NPC组织和细胞系中的表达。通过功能实验研究circ_ROBO1和/或miR-324-3p对NPC细胞增殖、迁移、侵袭和凋亡的调控作用。探究circ_ROBO1、miR-324-3p和NME1之间的相互作用。在小鼠体内研究肿瘤生长和转移情况。
结果
circ_ROBO在NPC中高表达。敲低circ_ROBO1可抑制NPC细胞的增殖、迁移和侵袭，并诱导其凋亡。circ_ROBO1缺失可减少小鼠肿瘤生长和转移。circ_ROBO1与miR-324-3p竞争上调NME1。降低miR-324-3p表达可削弱敲低circ_ROBO1对NPC细胞的作用。
结论
circ_ROBO1过表达通过调节miR-324-3p/NME1轴促进NPC进展。
Zotero 插件