Yao Liqin, Chu Chenghan, Li Yicheng, Cao Li, Yang Jianhua, Mu Wenbo
Department of Sports Medicine, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Bone Joint Res. 2024 Sep 3;13(9):441-451. doi: 10.1302/2046-3758.139.BJR-2023-0319.R3.
This study investigated vancomycin-microbubbles (Vm-MBs) and meropenem (Mp)-MBs with ultrasound-targeted microbubble destruction (UTMD) to disrupt biofilms and improve bactericidal efficiency, providing a new and promising strategy for the treatment of device-related infections (DRIs).
A film hydration method was used to prepare Vm-MBs and Mp-MBs and examine their characterization. Biofilms of methicillin-resistant (MRSA) and were treated with different groups. Biofilm biomass differences were determined by staining. Thickness and bacterial viability were observed with confocal laser scanning microscope (CLSM). Colony counts were determined by plate-counting. Scanning electron microscopy (SEM) observed bacterial morphology.
The Vm-MBs and Mp-MBs met the experimental requirements. The biofilm biomass in the Vm, Vm-MBs, UTMD, and Vm-MBs + UTMD groups was significantly lower than in the control group. MRSA and biofilms were most notably damaged in the Vm-MBs + UTMD group and Mp-MBs + UTMD group, respectively, with mean 21.55% (SD 0.08) and 19.73% (SD 1.25) remaining in the biofilm biomass. Vm-MBs + UTMD significantly reduced biofilm thickness and bacterial viability (p = 0.005 and p < 0.0001, respectively). Mp-MBs + UTMD could significantly decrease biofilm thickness and bacterial viability (allp < 0.001). Plate-counting method showed that the numbers of MRSA and bacterial colonies were significantly lower in the Vm-MBs + UTMD group and the Mp, Mp-MBs, UTMD, Mp-MBs + UTMD groups compared to the control group (p = 0.031). SEM showed that the morphology and structure of MRSA and were significantly damaged in the Vm-MBs + UTMD and Mp-MBs + UTMD groups.
Vm-MBs or Mp-MBs combined with UTMD can effectively disrupt biofilms and protectively release antibiotics under ultrasound mediation, significantly reducing bacterial viability and improving the bactericidal effect of antibiotics.
本研究调查了万古霉素微泡（Vm-MBs）和美罗培南（Mp）微泡联合超声靶向微泡破坏（UTMD）以破坏生物膜并提高杀菌效率，为治疗与装置相关的感染（DRIs）提供一种新的且有前景的策略。
采用薄膜水化法制备Vm-MBs和Mp-MBs并检测其特性。用不同组处理耐甲氧西林金黄色葡萄球菌（MRSA）和[此处原文缺失一种细菌名称]的生物膜。通过染色确定生物膜生物量差异。用共聚焦激光扫描显微镜（CLSM）观察厚度和细菌活力。通过平板计数法确定菌落数。扫描电子显微镜（SEM）观察细菌形态。
Vm-MBs和Mp-MBs符合实验要求。Vm组、Vm-MBs组、UTMD组和Vm-MBs + UTMD组的生物膜生物量显著低于对照组。MRSA和[此处原文缺失一种细菌名称]生物膜在Vm-MBs + UTMD组和Mp-MBs + UTMD组分别受到最显著破坏，生物膜生物量分别剩余平均21.55%（标准差0.08）和19.73%（标准差1.25）。Vm-MBs + UTMD显著降低生物膜厚度和细菌活力（分别为p = 0.005和p < 0.0001）。Mp-MBs + UTMD可显著降低生物膜厚度和细菌活力（所有p < 0.001）。平板计数法显示，与对照组相比，Vm-MBs + UTMD组以及Mp组、Mp-MBs组、UTMD组、Mp-MBs + UTMD组中MRSA和[此处原文缺失一种细菌名称]的菌落数显著更低（p = 0.031）。SEM显示，Vm-MBs + UTMD组和Mp-MBs + UTMD组中MRSA和[此处原文缺失一种细菌名称]的形态和结构受到显著破坏。
Vm-MBs或Mp-MBs联合UTMD可在超声介导下有效破坏生物膜并保护性释放抗生素，显著降低细菌活力并提高抗生素的杀菌效果。
