Pijls B G, Sanders Ingrid M J G, Kuijper Ed J, Nelissen R G H H
Department of Orthopaedics, Leiden University Medical Center, Leiden, The Netherlands.
Department of Medical Microbiology, Section Experimental Bacteriology, Leiden University Medical Center, Leiden, The Netherlands.
Bone Joint Res. 2022 Sep;11(9):629-638. doi: 10.1302/2046-3758.119.BJR-2022-0010.R1.
Here we used a mature seven-day biofilm model of exposed to antibiotics up to an additional seven days, to establish the effectiveness of either mechanical cleaning or antibiotics or non-contact induction heating, and which combinations could eradicate in mature biofilms.
Mature biofilms of (ATCC 29213) were grown on titanium alloy (Ti6Al4V) coupons for seven days and were subjected to the following treatments or their combinations: antibiotics, mechanical cleaning, or heat shock by induction heating of 60°C for one minute. Experiments were repeated at least five times.
In the untreated biofilm, growth up to 1.8×10 colony-forming units (CFU)/cm was observed. Treatment with ciprofloxacin, flucloxacillin, vancomycin, cefuroxime, and amoxicillin all with rifampicin gave 6.0 log, 6.1 log, 1.4 log, 4.8 log, and 3.6 log reduction in CFU/cm, respectively. Mechanical cleaning alone resulted in 4.9 log reduction and induction heating in 7.3 log reduction. There was an additional effect of ciprofloxacin, flucloxacillin, and induction heating when used in combinations. There was no additional effect for mechanical cleaning. No bacterial growth could be detected after induction heating followed by seven days of ciprofloxacin with rifampicin.
Mechanical cleaning, antibiotics, and non-contact induction heating reduced the bacterial load of mature biofilms with approximately 5 log or more as a single treatment. The effect of mechanical cleaning on mature biofilms was limited when used in combination with antibiotics and/or induction heating.Cite this article: 2022;11(9):629-638.
在此，我们使用成熟的7天生物膜模型，将其再暴露于抗生素长达7天，以确定机械清洁、抗生素或非接触感应加热的有效性，以及哪些组合可以根除成熟生物膜中的细菌。
金黄色葡萄球菌（ATCC 29213）的成熟生物膜在钛合金（Ti6Al4V）试片上生长7天，然后进行以下处理或其组合：抗生素、机械清洁或通过60°C感应加热1分钟进行热休克处理。实验至少重复5次。
在未处理的生物膜中，观察到生长至1.8×10菌落形成单位（CFU）/平方厘米。环丙沙星、氟氯西林、万古霉素、头孢呋辛和阿莫西林与利福平联合使用时，CFU/平方厘米分别降低了6.0对数、6.1对数、1.4对数、4.8对数和3.6对数。单独机械清洁导致CFU降低4.9对数，感应加热导致CFU降低7.3对数。环丙沙星、氟氯西林与感应加热联合使用时有额外效果。机械清洁没有额外效果。感应加热后再使用7天环丙沙星和利福平后未检测到细菌生长。
机械清洁、抗生素和非接触感应加热作为单一处理可使成熟金黄色葡萄球菌生物膜的细菌载量降低约5对数或更多。与抗生素和/或感应加热联合使用时，机械清洁对成熟金黄色葡萄球菌生物膜的效果有限。引用本文：2022；11（9）：629 - 638。
