Department of Chemistry, College of Science, United Arab Emirates University (UAEU), P.O. Box 15551, Al Ain, United Arab Emirates.
Internal Medicine, Faculty of Medicine and Health Sciences, United Arab Emirates University (UAEU), P.O. Box 15551, Al Ain, United Arab Emirates.
Sci Rep. 2024 Sep 2;14(1):20373. doi: 10.1038/s41598-024-71145-4.
Vitamin A plays a critical role in various biological functions, including vision, cellular differentiation, and immune regulation. However, accurately assessing its status, particularly in obese individuals, presents challenges due to potential alterations in metabolism and distribution. This study utilized Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) methodology to precisely measure serum vitamin A concentrations in population of UAE. The methodology's reliability and precision, as demonstrated through validation procedures, underscore its potential utility in clinical settings. Employing the Multiple Reaction Monitoring mode of positive ion electrospray ionization, the LC-MS/MS system achieves a limit of detection (LOD) of 0.48 ng/mL in serum, while adhering to FDA-US regulations for accuracy and compliance. A key aspect of this study was the application of LC-MS/MS to assess vitamin A status in an obese population within UAE. By employing a diverse cohort of 452 Emirati participants, including 277 individuals from a randomized controlled trial who were assessed at baseline and at 6th month, and 175 healthy individuals aged 18-82 assessed at baseline, this study explores the relationship between obesity and vitamin A levels, shedding light on potential implications for health and well-being. It was an observational study based on a new vitamin A method and participants were asked to eat vitamin A rich foods. The robust performance of the LC-MS/MS methodology positions it as a valuable tool for clinical research. By accurately quantifying vitamin A levels in human serum, this methodology opens avenues for advancing our understanding of vitamin A physiology and its implications for health, particularly in obese populations. In summary, this LC-MS/MS methodology presents a potent tool for clinical studies, providing reliable, specific, and robust detection of vitamin A in human serum, thus, opening a new frontier for advancing our understanding of vitamin A related physiology and health in the obese population.
维生素 A 在各种生物学功能中发挥着关键作用，包括视觉、细胞分化和免疫调节。然而，由于代谢和分布的潜在变化，准确评估其状态，特别是在肥胖个体中，具有挑战性。本研究采用液相色谱-串联质谱法（LC-MS/MS）对阿联酋人群的血清维生素 A 浓度进行了精确测量。该方法的可靠性和精密度通过验证程序得到了证明，突出了其在临床环境中的潜在应用价值。该 LC-MS/MS 系统采用正离子电喷雾电离的多反应监测模式，在血清中的检测限（LOD）为 0.48ng/mL，同时符合 FDA-US 关于准确性和合规性的规定。本研究的一个关键方面是应用 LC-MS/MS 评估阿联酋肥胖人群的维生素 A 状态。本研究采用了一个多样化的 452 名阿联酋参与者队列，包括 277 名来自随机对照试验的参与者，他们在基线和第 6 个月进行了评估，以及 175 名年龄在 18-82 岁的健康参与者在基线时进行了评估，该研究探讨了肥胖与维生素 A 水平之间的关系，为健康和福祉提供了潜在影响。这是一项基于新的维生素 A 方法的观察性研究，参与者被要求食用富含维生素 A 的食物。LC-MS/MS 方法的强大性能使其成为临床研究的有价值工具。通过准确地定量人类血清中的维生素 A 水平，该方法为我们深入了解维生素 A 生理学及其对健康的影响，特别是在肥胖人群中，开辟了新的途径。总之，这种 LC-MS/MS 方法为临床研究提供了一种有力的工具，为人类血清中维生素 A 的可靠、特异和稳健检测提供了可能，从而为我们深入了解肥胖人群中与维生素 A 相关的生理学和健康状况开辟了新的领域。
