生长激素使肝脏单加氧酶女性化的现象可被嘌呤霉素模拟，且与雄性型细胞色素P450的减少相关。

Feminization of the hepatic monooxygenases by growth hormone is mimicked by puromycin and correlates with a decrease in male-type cytochrome P450.

作者信息

Vockentanz B M, Virgo B B

出版信息

Biochem Biophys Res Commun. 1985 Apr 30;128(2):683-8. doi: 10.1016/0006-291x(85)90100-7.

Abstract

The hepatic monooxygenase system (MFO) was studied in hypophysectomized male rats treated with growth hormone (GH), puromycin, or both. GH significantly decreased the amount of cytochrome P450 and the activity of ethylmorphine demethylase but did not affect aniline hydroxylase or NADPH cytochrome c reductase. Puromycin significantly increased the activity of the reductase but otherwise had effects identical to GH. The agent's effects were additive. By labelling the P450 with [3H]-heme we found that GH decreased the amount of male-type (slow turnover) P450 by 56% but lowered the female-type (fast turnover) by only 10%. The hormone increased the half-life of both types by 56 and 100% respectively. We conclude that GH feminizes the MFO by decreasing the synthesis of male-type cytochrome P450.

摘要

对用生长激素（GH）、嘌呤霉素或两者处理的垂体切除雄性大鼠的肝脏单加氧酶系统（MFO）进行了研究。GH显著降低了细胞色素P450的量和乙基吗啡脱甲基酶的活性，但不影响苯胺羟化酶或NADPH细胞色素c还原酶。嘌呤霉素显著增加了还原酶的活性，但在其他方面具有与GH相同的作用。这些药物的作用是相加的。通过用[3H] - 血红素标记P450，我们发现GH使雄性型（周转缓慢）P450的量减少了56%，但使雌性型（周转快速）仅降低了10%。该激素分别使两种类型的半衰期增加了56%和100%。我们得出结论，GH通过减少雄性型细胞色素P450的合成使MFO女性化。