Roy J. Carver Department of Biomedical Engineering, College of Engineering, The University of Iowa, Iowa City, IA, USA.
Department of Anatomy and Cell Biology, Carver College of Medicine, The University of Iowa, Iowa City, IA, USA.
J Biomed Mater Res B Appl Biomater. 2024 Sep;112(9):e35476. doi: 10.1002/jbm.b.35476.
Mucoperiosteal wound healing, as it occurs after pediatric cleft palate surgery, can be challenging due to the limitations of current treatments such as tissue flaps secured with sutures and fibrin glue. In this study, we characterized the in vitro performance of a novel composite hydrogel biomaterial designed to be employed as an in situ wound filler and enhance mucoperiosteal wound healing. We evaluated a range of photopolymerizable formulations containing methacrylated gelatin (GelMA), glycol chitosan, and bioglass microparticles. Our aim was to identify one or more formulations with an appropriate balance of properties against a set of functional requirements that we established for this application. To test the formulations against these criteria, we measured photopolymerization kinetics, mechanical properties, degradation rate, in vitro biocompatibility, and ex vivo tissue adhesion. All formulations polymerized in less than 90 s using violet light. In addition, we found that GelMA-based hydrogels were more adhesive to mucoperiosteal tissue than clinical standard fibrin glue. Inclusion of small amounts of bioglass in the formulation increased mechanical compatibility with mucoperiosteal tissue, enhanced cytoconductivity, and promoted cell proliferation. Taken together, our results support the suitability of these photopolymerized composite hydrogels as in situ mucoperiosteal wound fillers. Overall, this study lays the groundwork for investigating the in vivo, pre-clinical effectiveness of these composite hydrogels in improving mucoperiosteal wound healing outcomes.
黏膜骨膜创面愈合，如在儿童腭裂手术后发生，由于目前治疗方法（如用缝线和纤维蛋白胶固定的组织瓣）的局限性，可能具有挑战性。在这项研究中，我们对一种新型复合水凝胶生物材料的体外性能进行了表征，该材料旨在用作原位创面填充剂，以增强黏膜骨膜创面愈合。我们评估了一系列含有甲基丙烯酰化明胶（GelMA）、壳聚糖乙二醇和生物玻璃微球的光聚合配方。我们的目的是确定一种或多种具有适当性能平衡的配方，以满足我们为该应用确定的一系列功能要求。为了根据这些标准测试配方，我们测量了光聚合动力学、机械性能、降解速率、体外生物相容性和离体组织粘附性。所有配方都在不到 90 秒的时间内使用紫光聚合。此外，我们发现基于 GelMA 的水凝胶比临床标准纤维蛋白胶更能粘附在黏膜骨膜组织上。配方中少量生物玻璃的加入提高了与黏膜骨膜组织的机械相容性，增强了细胞引导性，并促进了细胞增殖。总的来说，我们的结果支持这些光聚合复合水凝胶作为原位黏膜骨膜创面填充剂的适用性。总体而言，这项研究为研究这些复合水凝胶在改善黏膜骨膜创面愈合效果方面的体内、临床前有效性奠定了基础。
