载阿托伐他汀的羧甲基纤维素-明胶水凝胶：促进伤口愈合和皮肤组织再生的协同策略。

Atorvastatin-Loaded Carboxymethyl Cellulose-Gelatin Hydrogel: A Synergistic Strategy for Enhanced Wound Healing and Skin Tissue Regeneration.

作者信息

Mousavi Seyed Reza, Rashidi Mojtaba, Khedri Azam, Kouchak Maryam, Salehi Majid, Zamani Sepehr, Mohammadzadeh Ghorban

机构信息

Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Hyperlipidemia Research Center, Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

出版信息

Iran Biomed J. 2025 May 1;29(3):114-125. doi: 10.61186/ibj.5043.

DOI:10.61186/ibj.5043

PMID:40734372

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12396115/

Abstract

BACKGROUND

Skin tissue engineering is an innovative alternative to traditional methods for addressing skin injuries. This study aimed to synthesize a hydrogel consisting of carboxymethyl cellulose (CMC) and gelatin (Gel) containing atorvastatin (ATR) with the potential to accelerate tissue regeneration and wound healing in an animal model.

METHODS

Five unique formulations of hydrogel with different concentrations of ATR (0.1%, 0.5%, 1%, and 2% w/v) were synthesized using CMC-Gel. The structural characteristics of the hydrogels were assessed using SEM and FTIR spectroscopy. Additional evaluations carried out included swelling behavior, degradability, ATR release, compatibility, hemolytic activity, and the viability of NIH/3T3 fibroblast cells. The therapeutic effectiveness of these hydrogels in enhancing wound healing was investigated in an animal model by making a full-thickness skin incision in Wistar rats.

RESULTS

The synthesized CMC-Gel scaffolds had a porous structure with interconnected pores measuring 103 ± 8.74 μm and the ability to enhance cell migration. The MTT analysis showed a concentration-dependent relationship between ATR and cell proliferation, among which, the desirable concentration was 0.1% w/v. Furthermore, increased ATR concentrations were associated with decreased dressing capacity for hemostasis and coagulation. In vivo studies revealed that all the hydrogel-treated groups significantly outperformed the control group in promoting wound closure rates. Remarkably, the CMC-Gel-ATR 0.1% group exhibited the highest rates of wound closure, re-epithelialization, and angiogenesis.

CONCLUSION

Our results suggest the CMC-Gel-ATR as a desirable wound dressing for clinical application due to its unique physicochemical properties and comprehensive biocompatibility in in vitro and in vivo investigations.

摘要

背景

皮肤组织工程是解决皮肤损伤的传统方法的一种创新替代方案。本研究旨在合成一种由羧甲基纤维素（CMC）和明胶（Gel）组成并含有阿托伐他汀（ATR）的水凝胶，其具有在动物模型中加速组织再生和伤口愈合的潜力。

方法

使用CMC - Gel合成了五种不同ATR浓度（0.1%、0.5%、1%和2% w/v）的独特水凝胶配方。使用扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）对水凝胶的结构特征进行评估。还进行了其他评估，包括溶胀行为、降解性、ATR释放、相容性、溶血活性以及NIH/3T3成纤维细胞的活力。通过在Wistar大鼠身上制作全层皮肤切口，在动物模型中研究了这些水凝胶在促进伤口愈合方面的治疗效果。

结果

合成的CMC - Gel支架具有多孔结构，相互连通的孔隙大小为103±8.74μm，并且具有增强细胞迁移的能力。MTT分析显示ATR与细胞增殖之间存在浓度依赖性关系，其中理想浓度为0.1% w/v。此外，ATR浓度增加与止血和凝血的敷料能力降低有关。体内研究表明，所有水凝胶处理组在促进伤口闭合率方面均显著优于对照组。值得注意的是，0.1%的CMC - Gel - ATR组表现出最高的伤口闭合率、再上皮化率和血管生成率。