Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, China.
Clinical Medical Research Center, Affiliated Hospital of Nantong University, Nantong, China.
J Cell Biochem. 2020 Jan;121(1):557-565. doi: 10.1002/jcb.29296. Epub 2019 Jul 24.
Early diagnosis of gastric cancer (GC) is an effective method to improve prognosis. Increasing number of long noncoding RNAs (lncRNAs) have been reported as biomarkers for several cancers. We aim to detect the level of lncRNA B3GALT5-AS1 and its association with clinical parameters and to further explore its application value in GC. We measured serum B3GALT5-AS1 expression in 107 patients with GC, 40 polyp patients, and 87 normal controls to explore the significance of serum B3GALT5-AS1 in GC using the quantitative real-time polymerase chain reaction method. The result demonstrated that B3GALT5-AS1 level was markedly richer in GC patients than that in normal people (P < .001). B3GALT5-AS1 may be served as a diagnostic marker for distinguishing GC patients from healthy people, and the proportion under the receiver operating characteristics curve is 0.816 (95% confidence interval, 0.758-0.874; P = .03). Further exploration validated that high serum B3GALT5-AS1 level was related to TNM stage (P = .024), and lymph node metastasis (P = .023). Our study suggested that serum B3GALT5-AS1 may be employed as an ideal biomarker for early screening of GC.
早期诊断胃癌（GC）是改善预后的有效方法。越来越多的长链非编码 RNA（lncRNA）已被报道为几种癌症的生物标志物。我们旨在检测 lncRNA B3GALT5-AS1 的水平及其与临床参数的关系，并进一步探讨其在 GC 中的应用价值。我们使用定量实时聚合酶链反应方法测量了 107 例 GC 患者、40 例息肉患者和 87 名正常对照者的血清 B3GALT5-AS1 表达，以探讨血清 B3GALT5-AS1 在 GC 中的意义。结果表明，GC 患者的 B3GALT5-AS1 水平明显高于正常人（P < .001）。B3GALT5-AS1 可能作为区分 GC 患者与健康人的诊断标志物，受试者工作特征曲线下的比例为 0.816（95%置信区间，0.758-0.874；P = .03）。进一步的探索验证了高血清 B3GALT5-AS1 水平与 TNM 分期（P = .024）和淋巴结转移（P = .023）有关。我们的研究表明，血清 B3GALT5-AS1 可作为 GC 早期筛查的理想生物标志物。
