Zuo Wenjie, Yin Guobin, Zhang Luyao, Zhang Weijiao, Xu Ruirui, Wang Yang, Li Jianghua, Kang Zhen
The Science Center for Future Foods, Jiangnan University, Wuxi, 214122, China.
The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Synth Syst Biotechnol. 2024 Aug 8;10(1):49-57. doi: 10.1016/j.synbio.2024.08.003. eCollection 2025.
As a fundamental tool in synthetic biology, promoters are pivotal in regulating gene expression, enabling precise genetic control and spurring innovation across diverse biotechnological applications. However, most advances in engineered genetic systems rely on host-specific regulation of the genetic portion. With the burgeoning diversity of synthetic biology chassis cells, there emerges a pressing necessity to broaden the universal promoter toolkit spectrum, ensuring adaptability across various microbial chassis cells for enhanced applicability and customization in the evolving landscape of synthetic biology. In this study, we analyzed and validated the primary structures of natural endogenous promoters from , , , , and , and through strategic integration and rational modification of promoter motifs, we developed a series of cross-species promoters (P) with transcriptional activity in five strains (prokaryotic and eukaryotic). This series of cross species promoters can significantly expand the synthetic biology promoter toolkit while providing a foundation and inspiration for standardized development of universal components The combinatorial use of key elements from prokaryotic and eukaryotic promoters presented in this study represents a novel strategy that may offer new insights and methods for future advancements in promoter engineering.
作为合成生物学的基本工具，启动子在调节基因表达方面起着关键作用，能够实现精确的基因控制，并推动各种生物技术应用的创新。然而，工程遗传系统的大多数进展都依赖于对遗传部分的宿主特异性调控。随着合成生物学底盘细胞的多样性迅速增加，迫切需要拓宽通用启动子工具包的范围，以确保在各种微生物底盘细胞中具有适应性，从而在不断发展的合成生物学领域提高适用性和定制性。在本研究中，我们分析并验证了来自[具体物种1]、[具体物种2]、[具体物种3]、[具体物种4]和[具体物种5]的天然内源启动子的一级结构，并通过对启动子基序的策略性整合和合理修饰，开发了一系列在五种菌株（原核和真核）中具有转录活性的跨物种启动子（P）。这一系列跨物种启动子可以显著扩展合成生物学启动子工具包，同时为通用组件的标准化开发提供基础和灵感。本研究中提出的原核和真核启动子关键元件的组合使用代表了一种新策略，可能为启动子工程的未来进展提供新的见解和方法。
