利用巴斯德毕赤酵母对 C1 饲料原料进行生物转化以生产化学品。

Bioconversion of C1 feedstocks for chemical production using Pichia pastoris.

机构信息

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211800, P.R. China.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211800, P.R. China; Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing, 211800, P.R. China.

出版信息

Trends Biotechnol. 2023 Aug;41(8):1066-1079. doi: 10.1016/j.tibtech.2023.03.006. Epub 2023 Mar 24.

DOI:10.1016/j.tibtech.2023.03.006

PMID:36967258

Abstract

Bioconversion of C1 feedstocks for chemical production offers a promising solution to global challenges such as the energy and food crises and climate change. The methylotroph Pichia pastoris is an attractive host system for the production of both recombinant proteins and chemicals from methanol. Recent studies have also demonstrated its potential for utilizing CO through metabolic engineering or coupling with electrocatalysis. This review focuses on the bioconversion of C1 feedstocks for chemical production using P. pastoris. Herein the challenges and feasible strategies for chemical production in P. pastoris are discussed. The potential of P. pastoris to utilize other C1 feedstocks - including CO and formate - is highlighted, and new insights from the perspectives of synthetic biology and material science are proposed.

摘要

利用 C1 原料进行生物转化，为解决能源和粮食危机、气候变化等全球性挑战提供了一个有前景的方案。甲醇营养型酵母毕赤酵母是一种很有吸引力的宿主系统，可用于生产重组蛋白和甲醇衍生的化学品。最近的研究还表明，它具有通过代谢工程或与电催化耦合利用 CO 的潜力。本综述重点介绍了利用毕赤酵母进行 C1 原料生物转化以生产化学品。本文讨论了毕赤酵母在化学品生产方面面临的挑战和可行的策略。强调了毕赤酵母利用其他 C1 原料（包括 CO 和甲酸盐）的潜力，并从合成生物学和材料科学的角度提出了新的见解。