Gmeiner B M
Biochim Biophys Acta. 1985 May 20;829(1):76-82. doi: 10.1016/0167-4838(85)90070-6.
As analyzed by a phase-separation technique, the Triton X-114 extract of human mammary carcinoma cells (MCF-7 cells) contain an amphiphilic form of galactosyltransferase (UDPgalactose: D-glucose 4-beta-D-galactosyltransferase, EC 2.4.1.22), while the galactosyltransferase activity released by these cells represents a hydrophilic form of the enzyme. When the amphiphilic galactosyltransferase was subjected to limited proteolysis with thermolysin, this treatment generated a hydrophilic form of the enzyme. With respect to Km for UDPgalactose the kinetic data were very similar for the amphiphilic, for the released and the hydrophilic galactosyltransferases produced by proteinase treatment. Differences were detected in electrophoretic and gel chromatographic properties. The hydrophilic enzymes showed a greater electrophoretic mobility on non-denaturing polyacrylamide gels than did the amphiphilic form. On Sepharose 6B column chromatography, the amphiphilic galactosyltransferase appeared to be of higher molecular weight than the hydrophilic enzyme.
通过相分离技术分析，人乳腺癌细胞（MCF - 7细胞）的Triton X - 114提取物含有一种两亲性形式的半乳糖基转移酶（UDP - 半乳糖：D - 葡萄糖4 - β - D - 半乳糖基转移酶，EC 2.4.1.22），而这些细胞释放的半乳糖基转移酶活性代表了该酶的一种亲水性形式。当用嗜热菌蛋白酶对两亲性半乳糖基转移酶进行有限的蛋白水解时，这种处理产生了该酶的一种亲水性形式。就UDP - 半乳糖的Km而言，两亲性、释放的以及蛋白酶处理产生的亲水性半乳糖基转移酶的动力学数据非常相似。在电泳和凝胶色谱性质方面检测到了差异。亲水性酶在非变性聚丙烯酰胺凝胶上的电泳迁移率比两亲性形式的酶更高。在琼脂糖6B柱色谱上，两亲性半乳糖基转移酶的分子量似乎比亲水性酶更高。
