Juli Mst Sogra Banu, Raza Ali, Forutan Mehrnush, Siddle Hannah V, Fordyce Geoffry, Muller Jarud, Boe-Hansen Gry B, Tabor Ala E
Centre for Animal Science, The University of Queensland, Queensland Alliance for Agriculture and Food Innovation (QAAFI), Saint Lucia, QLD, Australia.
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.
Front Microbiol. 2024 Aug 19;15:1404525. doi: 10.3389/fmicb.2024.1404525. eCollection 2024.
Bovine genital campylobacteriosis (BGC) is a globally important venereal disease of cattle caused by subspecies . Diagnosis of BGC is highly challenging due to the lack of accurate diagnostic tests.
To characterise the biomarkers for infection, a total of twelve cycling heifers were selected and categorised as vaccinated ( = 6) with Vibrovax® (Zoetis™) and unvaccinated ( = 6). All heifers were oestrous synchronised with a double dose of prostaglandin (PGF2α) 11 days apart and when in oestrous intravaginally challenged with 2.7 x 10 CFU live . DNA extracted from vaginal mucus samples was screened using a qPCR and 16S rRNA was characterised using Illumina sequencing (V5-V8 region). Relative abundances of serum proteins were calculated using sequential window acquisition of all theoretical fragment ion spectra coupled to tandem mass spectrometry (SWATH-MS) for all heifers at three timepoints: pre-challenge, post-challenge and post-recovery.
In 16S rRNA sequencing of vaginal mucus, spp. appeared two days following challenge in unvaccinated compared to 14 days in vaccinated animals, consistent with the qPCR results. Increased relative abundances of Firmicutes and Campylobacterota were identified after challenge and were associated with in vaccinated and unvaccinated heifers. Greater relative abundance of spp. was observed during oestrous rather than dioestrous. In both vaccinated and unvaccinated heifers, spp. increased after challenge with higher abundance of spp. in the vaccinated group. A total of 130 unique proteins were identified in SWATH analysis of the serum samples, and the number of differentially abundant proteins found was higher in the vaccinated group after recovery from infection compared to pre-and post-challenge (adjusted < 0.05 and Log2FC > 0.2).
Coglutinin, clusterin, HP homologs, vitamin D binding protein and fetuin B were identified as potential biomarkers for infection and need further study to validate their efficiency as immune biomarkers for BGC.
牛生殖道弯曲杆菌病（BGC）是一种由亚种引起的全球重要的牛性病。由于缺乏准确的诊断测试，BGC的诊断极具挑战性。
为了鉴定感染的生物标志物，总共选择了12头处于发情周期的小母牛，并分为接种了Vibrovax®（硕腾公司）的接种组（n = 6）和未接种组（n = 6）。所有小母牛均用双倍剂量的前列腺素（PGF2α）进行发情同步，间隔11天，发情时经阴道接种2.7×10CFU活的[弯曲杆菌名称未给出]。从阴道黏液样本中提取的DNA用定量聚合酶链反应（qPCR）进行筛选，16S核糖体RNA（rRNA）用Illumina测序（V5 - V8区域）进行鉴定。在三个时间点（攻毒前、攻毒后和恢复后），对所有小母牛使用数据依赖型采集串联质谱（SWATH - MS）计算血清蛋白的相对丰度。
在阴道黏液的16S rRNA测序中，未接种组攻毒后2天出现[弯曲杆菌名称未给出]，而接种组为14天，这与qPCR结果一致。攻毒后，在接种和未接种的小母牛中均鉴定出厚壁菌门和弯曲杆菌门的相对丰度增加，且与[弯曲杆菌名称未给出]有关。发情期而非间情期观察到[弯曲杆菌名称未给出]的相对丰度更高。在接种和未接种的小母牛中，攻毒后[弯曲杆菌名称未给出]均增加，接种组中[弯曲杆菌名称未给出]的丰度更高。在血清样本的SWATH分析中总共鉴定出130种独特的蛋白质，与攻毒前和攻毒后相比，感染恢复后的接种组中发现的差异丰富蛋白质数量更多（校正P < 0.05且Log2倍变化> 0.2）。
凝集素、簇集素、HP同源物、维生素D结合蛋白和胎球蛋白B被鉴定为[弯曲杆菌名称未给出]感染的潜在生物标志物，需要进一步研究以验证它们作为BGC免疫生物标志物的有效性。
