Wu Jiayan, Yu Jiandong, Zhu Hongquan, Chen Zhiping, Liang Yongling, Chen Qin, Li Guolin, Wan Yunle
Department of General Surgery (Hepatobiliary, Pancreatic and Splenic Surgery), The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, China.
Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, China.
Heliyon. 2024 Aug 8;10(16):e35906. doi: 10.1016/j.heliyon.2024.e35906. eCollection 2024 Aug 30.
LncRNA HOXB-AS3 are associated with tumor progression in several types of carcinomas, yet, its possibly biological role in gallbladder carcinoma(GBC) remains unclear. Therefore, this study aimed to investigate the biological function of HOXB-AS3 in GBC.
To know the potential function of HOXB-AS3 in gallbladder carcinoma, real-time polymerase chain reaction was used to detected the expression of HOXB-AS3 in gallbladder carcinoma cells. The colony formation assay and cell counting kit-8 assay was performed to measured cell viability. Flow cytometry was to analyse cell apoptosis and cell cycle. Cell invasion and migration were determined by the transwell invasion assay and wound-healing assay. A nude mice xenograft tumor model was performed to investigate the biological function of HOXB-AS3 in vivo.
The results indicated that HOXB-AS3 was significantly elevated in gallbladder carcinoma tissues and cell lines. We used siHOXB-AS3 to knockdown the expression levels of HOXB-AS3. And knockdown HOXB-AS3 expression depressed gallbladder cancer cell viability and induced cell apoptosis. In addition, the gallbladder carcinoma cell cycle was obviously arrested at the G1 phase. Cell invasion and migration were markedly suppressed following knockdown HOXB-AS3 expression. Furthermore, the features of siHOXB-AS3 in gallbladder cancer cells could be reversed by the ERK1/2 phosphorylation agonist Ro 67-7476. Finally, we confirmed that HOXB-AS3 promoted the growth of transplanted tumors in vivo.
HOXB-AS3 promoted gallbladder carcinoma cell proliferation, invasion and migration by activating the MEK/ERK signaling pathway. HOXB-AS3 contributed to gallbladder cancer tumorigenesis and metastasis, making it a viable therapeutic target for gallbladder cancer treatment.
长链非编码RNA HOXB-AS3与多种类型的癌症肿瘤进展相关，然而，其在胆囊癌（GBC）中可能的生物学作用仍不清楚。因此，本研究旨在探讨HOXB-AS3在GBC中的生物学功能。
为了解HOXB-AS3在胆囊癌中的潜在功能，采用实时聚合酶链反应检测胆囊癌细胞中HOXB-AS3的表达。进行集落形成试验和细胞计数试剂盒-8试验以测量细胞活力。采用流式细胞术分析细胞凋亡和细胞周期。通过Transwell侵袭试验和伤口愈合试验测定细胞侵袭和迁移能力。建立裸鼠异种移植瘤模型以研究HOXB-AS3在体内的生物学功能。
结果表明，HOXB-AS3在胆囊癌组织和细胞系中显著升高。我们使用siHOXB-AS3敲低HOXB-AS3的表达水平。敲低HOXB-AS3表达可抑制胆囊癌细胞活力并诱导细胞凋亡。此外，胆囊癌细胞周期明显停滞在G1期。敲低HOXB-AS3表达后，细胞侵袭和迁移能力明显受到抑制。此外，ERK1/2磷酸化激动剂Ro 67-7476可逆转胆囊癌细胞中siHOXB-AS3的特征。最后，我们证实HOXB-AS3在体内促进移植瘤生长。
HOXB-AS3通过激活MEK/ERK信号通路促进胆囊癌细胞增殖、侵袭和迁移。HOXB-AS3促进胆囊癌的肿瘤发生和转移，使其成为胆囊癌治疗的一个可行的治疗靶点。
