• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码RNA（lncRNA）HOXB-AS3通过靶向miR-498-5p调控ADAM9的表达，从而促进子宫内膜癌细胞增殖并抑制其凋亡。

Long non-coding RNA (lncRNA) HOXB-AS3 promotes cell proliferation and inhibits apoptosis by regulating ADAM9 expression through targeting miR-498-5p in endometrial carcinoma.

作者信息

Xing Ying, Sun Xianhua, Li Feng, Jiang Xuan, Jiang Afang, Li Xiaofan, Lv Ruiting, Shao Liwei

机构信息

Department of General Medicine, Wulidun Neighborhood Community Health Service Center Affiliated with the Fifth Hospital of Wuhan, Wuhan, Hubei, China.

Department of Infectious Disease, Red Cross Society Hospital of Wuhan (Wuhan No.11 Hospital), Wuhan, Hubei, China.

出版信息

J Int Med Res. 2021 Jun;49(6):3000605211013548. doi: 10.1177/03000605211013548.

DOI:10.1177/03000605211013548
PMID:34187214
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8258772/
Abstract

OBJECTIVE

Long non-coding RNA (lncRNA) expression is closely related to the pathogenesis and progression of various tumors. In this study, we investigated the mechanisms of lncRNA HOXB cluster antisense RNA 3 (HOXB-AS3), miRNA(miR)-498-5p, and disintegrin and metalloproteinase domain-containing protein 9 (ADAM9) in endometrial carcinoma (EC) cells.

METHODS

The expression levels of lncRNA HOXB-AS3 in EC tissues and cells were detected using RT-qPCR assays. The effects of HOXB-AS3 knockdown on EC cell proliferation and apoptosis were measured using CCK-8 assays, colony formation assays, and flow cytometry. In addition, putative miR-498-5p binding sites were identified in HOXB-AS3 and ADAM9. The targeted relationships were further verified using dual-luciferase reporter and RNA pull-down assays.

RESULTS

HOXB-AS3 expression was upregulated in EC tissues and cells. EC cell proliferation and viability decreased significantly in HOXB-AS3 knockdown groups. A putative miR-498-5p binding site in HOXB-AS3 was verified. Inhibition of miR-498-5p rescued the effects of HOXB-AS3 knockdown on cell proliferation and apoptosis. Finally, ADAM9 was verified as a direct target gene of miR-498-5p.

CONCLUSIONS

Our results suggest that lncRNA HOXB-AS3 is highly expressed in EC tissues and cells. Downregulation of HOXB-AS3 inhibits cell proliferation and promotes apoptosis in EC cells. HOXB-AS3 can upregulate ADAM9 expression by sponging miR-498-5p.

摘要

目的

长链非编码RNA（lncRNA）表达与多种肿瘤的发病机制和进展密切相关。在本研究中，我们探讨了lncRNA HOXB簇反义RNA 3（HOXB-AS3）、微小RNA（miR）-498-5p和含去整合素和金属蛋白酶结构域蛋白9（ADAM9）在子宫内膜癌（EC）细胞中的作用机制。

方法

采用RT-qPCR检测EC组织和细胞中lncRNA HOXB-AS3的表达水平。采用CCK-8法、集落形成法和流式细胞术检测HOXB-AS3敲低对EC细胞增殖和凋亡的影响。此外，在HOXB-AS3和ADAM9中鉴定了假定的miR-498-5p结合位点。通过双荧光素酶报告基因和RNA下拉实验进一步验证靶向关系。

结果

HOXB-AS3在EC组织和细胞中表达上调。HOXB-AS3敲低组EC细胞增殖和活力显著降低。验证了HOXB-AS3中一个假定的miR-498-5p结合位点。抑制miR-498-5p可挽救HOXB-AS3敲低对细胞增殖和凋亡的影响。最后，验证ADAM9是miR-498-5p的直接靶基因。

结论

我们的结果表明，lncRNA HOXB-AS3在EC组织和细胞中高表达。HOXB-AS3的下调抑制EC细胞增殖并促进其凋亡。HOXB-AS3可通过海绵吸附miR-498-5p上调ADAM9表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4272/8258772/450b232b0403/10.1177_03000605211013548-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4272/8258772/7422d71e2f82/10.1177_03000605211013548-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4272/8258772/263fe84610f6/10.1177_03000605211013548-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4272/8258772/265fa90aade7/10.1177_03000605211013548-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4272/8258772/b7f59dca4e4a/10.1177_03000605211013548-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4272/8258772/450b232b0403/10.1177_03000605211013548-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4272/8258772/7422d71e2f82/10.1177_03000605211013548-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4272/8258772/263fe84610f6/10.1177_03000605211013548-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4272/8258772/265fa90aade7/10.1177_03000605211013548-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4272/8258772/b7f59dca4e4a/10.1177_03000605211013548-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4272/8258772/450b232b0403/10.1177_03000605211013548-fig5.jpg

相似文献

1
Long non-coding RNA (lncRNA) HOXB-AS3 promotes cell proliferation and inhibits apoptosis by regulating ADAM9 expression through targeting miR-498-5p in endometrial carcinoma.长链非编码RNA（lncRNA）HOXB-AS3通过靶向miR-498-5p调控ADAM9的表达，从而促进子宫内膜癌细胞增殖并抑制其凋亡。
J Int Med Res. 2021 Jun;49(6):3000605211013548. doi: 10.1177/03000605211013548.
2
LncRNA HOXB-AS3 binding to PTBP1 protein regulates lipid metabolism by targeting SREBP1 in endometrioid carcinoma.长链非编码RNA HOXB-AS3与PTBP1蛋白结合通过靶向子宫内膜样腺癌中的SREBP1来调节脂质代谢。
Life Sci. 2023 May 1;320:121512. doi: 10.1016/j.lfs.2023.121512. Epub 2023 Feb 27.
3
Long non-coding RNA HOXA-AS3 promotes cell proliferation of oral squamous cell carcinoma through sponging microRNA miR-218-5p.长链非编码 RNA HOXA-AS3 通过海绵吸附 microRNA miR-218-5p 促进口腔鳞状细胞癌的细胞增殖。
Bioengineered. 2021 Dec;12(1):8724-8737. doi: 10.1080/21655979.2021.1978196.
4
LncRNA HOXB-AS3 promotes growth, invasion and migration of epithelial ovarian cancer by altering glycolysis.长链非编码 RNA HOXB-AS3 通过改变糖酵解促进上皮性卵巢癌的生长、侵袭和迁移。
Life Sci. 2021 Jan 1;264:118636. doi: 10.1016/j.lfs.2020.118636. Epub 2020 Oct 24.
5
lncRNA NBAT1 Inhibits Cell Metastasis and Promotes Apoptosis in Endometrial Cancer by Sponging miR-21-5p to Regulate PTEN.长链非编码 RNA NBAT1 通过海绵吸附 miR-21-5p 调控 PTEN 抑制子宫内膜癌细胞转移并促进细胞凋亡。
Comput Math Methods Med. 2022 Jul 20;2022:9304392. doi: 10.1155/2022/9304392. eCollection 2022.
6
LncRNA LOXL1-AS1 promotes endometrial cancer progression by sponging miR-28-5p to upregulate RAP1B expression.长链非编码 RNA LOXL1-AS1 通过海绵吸附 miR-28-5p 来上调 RAP1B 表达，促进子宫内膜癌进展。
Biomed Pharmacother. 2020 May;125:109839. doi: 10.1016/j.biopha.2020.109839. Epub 2020 Jan 29.
7
Overexpression of long noncoding RNA HOXB-AS3 indicates an unfavorable prognosis and promotes tumorigenesis in epithelial ovarian cancer via Wnt/β-catenin signaling pathway.长链非编码RNA HOXB-AS3的过表达提示上皮性卵巢癌预后不良，并通过Wnt/β-连环蛋白信号通路促进肿瘤发生。
Biosci Rep. 2019 Aug 2;39(8). doi: 10.1042/BSR20190906. Print 2019 Aug 30.
8
Knockdown of circ-ADAM9 inhibits malignant phenotype and enhances radiosensitivity in breast cancer cells via acting as a sponge for miR-383-5p.环状-ADAM9的敲低通过充当miR-383-5p的海绵来抑制乳腺癌细胞的恶性表型并增强其放射敏感性。
Strahlenther Onkol. 2023 Jan;199(1):78-89. doi: 10.1007/s00066-022-02006-0. Epub 2022 Oct 7.
9
lncRNA HOXB-AS3 promotes hepatoma by inhibiting p53 expression.长链非编码 RNA HOXB-AS3 通过抑制 p53 表达促进肝癌。
Eur Rev Med Pharmacol Sci. 2018 Oct;22(20):6784-6792. doi: 10.26355/eurrev_201810_16145.
10
LncRNA HOXA-AS3 promotes the malignancy of glioblastoma through regulating miR-455-5p/USP3 axis.长链非编码 RNA HOXA-AS3 通过调控 miR-455-5p/USP3 轴促进胶质母细胞瘤的恶性进展。
J Cell Mol Med. 2020 Oct;24(20):11755-11767. doi: 10.1111/jcmm.15788. Epub 2020 Sep 11.

引用本文的文献

1
Building a Hand-Curated ceRNET for Endometrial Cancer, Striving for Clinical as Well as Medicolegal Soundness: A Systematic Review.构建子宫内膜癌人工筛选的ceRNET，追求临床及法医学合理性：一项系统评价
Noncoding RNA. 2025 Apr 30;11(3):34. doi: 10.3390/ncrna11030034.
2
The diagnostic and prognostic significance of HOXC13-AS and its molecular regulatory mechanism in human cancer.HOXC13-AS在人类癌症中的诊断和预后意义及其分子调控机制
Front Mol Biosci. 2025 Feb 6;12:1540048. doi: 10.3389/fmolb.2025.1540048. eCollection 2025.
3
LncRNA HOXB-AS3 promotes proliferation, migration, and invasion of gallbladder cancer cells by activating the MEK/ERK pathway.

本文引用的文献

1
LncRNA HOXB-AS3 promotes growth, invasion and migration of epithelial ovarian cancer by altering glycolysis.长链非编码 RNA HOXB-AS3 通过改变糖酵解促进上皮性卵巢癌的生长、侵袭和迁移。
Life Sci. 2021 Jan 1;264:118636. doi: 10.1016/j.lfs.2020.118636. Epub 2020 Oct 24.
2
Silencing lncRNA AFAP1-AS1 Inhibits the Progression of Esophageal Squamous Cell Carcinoma Cells via Regulating the miR-498/VEGFA Axis.沉默长链非编码RNA AFAP1-AS1通过调控miR-498/VEGFA轴抑制食管鳞状细胞癌细胞的进展。
Cancer Manag Res. 2020 Jul 29;12:6397-6409. doi: 10.2147/CMAR.S254302. eCollection 2020.
3
LncRNA LINC00689 Promotes the Progression of Gastric Cancer Through Upregulation of ADAM9 by Sponging miR-526b-3p.
长链非编码RNA HOXB-AS3通过激活MEK/ERK信号通路促进胆囊癌细胞的增殖、迁移和侵袭。
Heliyon. 2024 Aug 8;10(16):e35906. doi: 10.1016/j.heliyon.2024.e35906. eCollection 2024 Aug 30.
4
Exploring the Dark Matter of Human Proteome: The Emerging Role of Non-Canonical Open Reading Frame (ncORF) in Cancer Diagnosis, Biology, and Therapy.探索人类蛋白质组的暗物质：非规范开放阅读框（ncORF）在癌症诊断、生物学及治疗中的新兴作用
Cancers (Basel). 2024 Jul 26;16(15):2660. doi: 10.3390/cancers16152660.
5
The Promotive and Inhibitory Role of Long Non-Coding RNAs in Endometrial Cancer Course-A Review.长链非编码RNA在子宫内膜癌病程中的促进和抑制作用——综述
Cancers (Basel). 2024 Jun 3;16(11):2125. doi: 10.3390/cancers16112125.
6
Advances in MiRNAs Involved in Endometrial Carcinoma.参与子宫内膜癌的微小RNA的研究进展
Comb Chem High Throughput Screen. 2025;28(1):3-11. doi: 10.2174/0113862073299444240308145725.
7
A concise review of the regulatory, diagnostic, and prognostic implications of HOXB-AS3 in tumors.HOXB-AS3在肿瘤中的调控、诊断及预后意义的简要综述
J Cancer. 2024 Jan 1;15(3):714-728. doi: 10.7150/jca.91033. eCollection 2024.
8
lncRNA NBAT1 Inhibits Cell Metastasis and Promotes Apoptosis in Endometrial Cancer by Sponging miR-21-5p to Regulate PTEN.长链非编码 RNA NBAT1 通过海绵吸附 miR-21-5p 调控 PTEN 抑制子宫内膜癌细胞转移并促进细胞凋亡。
Comput Math Methods Med. 2022 Jul 20;2022:9304392. doi: 10.1155/2022/9304392. eCollection 2022.
9
Emerging Roles of lncRNAs Regulating RNA-Mediated Type-I Interferon Signaling Pathway.长链非编码 RNA 调控 RNA 介导的 I 型干扰素信号通路的新兴作用。
Front Immunol. 2022 Feb 25;13:811122. doi: 10.3389/fimmu.2022.811122. eCollection 2022.
10
Functional Peptides Encoded by Long Non-Coding RNAs in Gastrointestinal Cancer.长链非编码RNA编码的功能性肽在胃肠道癌中的作用
Front Oncol. 2021 Nov 23;11:777374. doi: 10.3389/fonc.2021.777374. eCollection 2021.
长链非编码RNA LINC00689通过海绵化miR-526b-3p上调ADAM9促进胃癌进展。
Cancer Manag Res. 2020 Jun 4;12:4227-4239. doi: 10.2147/CMAR.S231042. eCollection 2020.
4
circ_0000638 inhibits neodymium oxide-induced bronchial epithelial cell inflammation through the miR-498-5p/NF-κB axis.环状 RNA 0000638 通过 miR-498-5p/NF-κB 轴抑制氧化钕诱导的支气管上皮细胞炎症。
Ecotoxicol Environ Saf. 2020 Jun 1;195:110455. doi: 10.1016/j.ecoenv.2020.110455. Epub 2020 Mar 18.
5
The long non-coding RNA HOXB-AS3 regulates ribosomal RNA transcription in NPM1-mutated acute myeloid leukemia.长链非编码 RNA HOXB-AS3 调控 NPM1 突变型急性髓系白血病的核糖体 RNA 转录。
Nat Commun. 2019 Nov 25;10(1):5351. doi: 10.1038/s41467-019-13259-2.
6
Downregulation of hsa_circ_0007534 restricts the proliferation and invasion of cervical cancer through regulating miR-498/BMI-1 signaling.hsa_circ_0007534 的下调通过调节 miR-498/BMI-1 信号通路限制宫颈癌的增殖和侵袭。
Life Sci. 2019 Oct 15;235:116785. doi: 10.1016/j.lfs.2019.116785. Epub 2019 Aug 21.
7
Overexpression of long noncoding RNA HOXB-AS3 indicates an unfavorable prognosis and promotes tumorigenesis in epithelial ovarian cancer via Wnt/β-catenin signaling pathway.长链非编码RNA HOXB-AS3的过表达提示上皮性卵巢癌预后不良，并通过Wnt/β-连环蛋白信号通路促进肿瘤发生。
Biosci Rep. 2019 Aug 2;39(8). doi: 10.1042/BSR20190906. Print 2019 Aug 30.
8
Long non-coding RNA HOXB-AS3 promotes myeloid cell proliferation and its higher expression is an adverse prognostic marker in patients with acute myeloid leukemia and myelodysplastic syndrome.长非编码 RNA HOXB-AS3 促进髓系细胞增殖，其高表达是急性髓系白血病和骨髓增生异常综合征患者的不良预后标志物。
BMC Cancer. 2019 Jun 24;19(1):617. doi: 10.1186/s12885-019-5822-y.
9
Regulatory roles of long noncoding RNAs implicated in cancer hallmarks.长非编码 RNA 在癌症特征中的调控作用。
Int J Cancer. 2020 Feb 15;146(4):906-916. doi: 10.1002/ijc.32277. Epub 2019 Apr 1.
10
lncRNA HOXB-AS3 promotes hepatoma by inhibiting p53 expression.长链非编码 RNA HOXB-AS3 通过抑制 p53 表达促进肝癌。
Eur Rev Med Pharmacol Sci. 2018 Oct;22(20):6784-6792. doi: 10.26355/eurrev_201810_16145.