Ahlsten G, Ewald U, Tuvemo T
Biol Neonate. 1985;47(4):199-204. doi: 10.1159/000242116.
Using a double-sample technique, platelet aggregation induced by arachidonic acid (AA) was studied in cord blood samples from 29 newborns and 20 adults, all healthy and drug-free. Of the platelets from adults (10 males, 10 females), all aggregated after 1.0 mM AA. After 0.5 mM AA, all platelets aggregated except those from 4 males which did not respond at all. The great majority of cord blood samples aggregated similarly to samples from adults. Platelets from all 15 newborn females and 11 of 14 newborn males aggregated irreversibly after 1.0 mM AA, while those from 3 of the 14 male newborns showed a reversible aggregation. All 11 newborn samples aggregated irreversibly after 0.5 mM AA. These results prove that the cyclo-oxygenase pathway for platelet aggregation operates satisfactorily in newborn infants. The double-sample technique is of value for cord blood samples. Late cord blood samples showed more marked aggregation than samples taken earlier.
采用双样本技术，对29名新生儿和20名成年人的脐血样本中花生四烯酸（AA）诱导的血小板聚集进行了研究，所有受试者均健康且未服用药物。在成年人的血小板中（10名男性，10名女性），1.0 mM AA作用后所有血小板均发生聚集。0.5 mM AA作用后，除4名男性的血小板完全无反应外，其他所有血小板均发生聚集。绝大多数脐血样本的聚集情况与成年人样本相似。15名新生儿女性和14名新生儿男性中的11名的血小板在1.0 mM AA作用后发生不可逆聚集，而14名男性新生儿中的3名表现为可逆性聚集。11份新生儿样本在0.5 mM AA作用后均发生不可逆聚集。这些结果证明血小板聚集的环氧化酶途径在新生儿中运作良好。双样本技术对脐血样本很有价值。晚期脐血样本的聚集比早期采集的样本更明显。
