Clemmons R M, Meyers K M
Am J Vet Res. 1984 Jan;45(1):137-44.
A method for obtaining reliable blood platelet yields in canine platelet-rich plasma, using increased sodium citrate concentration, is presented. Maintaining a quiet environment or anesthetizing the animals with thiamylal sodium aids in collection of platelets. Aggregation of platelets from 60 dogs of various breeds in response to arachidonic acid, collagen, adenosine diphosphate, epinephrine, and serotonin was monitored. Canine platelets reversibly or irreversibly aggregated to arachidonic acid. The percentage of arachidonate-irreversible platelets varied from 0% to 100% depending upon the breed of dogs examined. Arachidonate-irreversible platelets also aggregated irreversibly at lower concentrations of collagen and exhibited biphasic irreversible aggregation to adenosine diphosphate and serotonin. Serotonin-induced irreversible aggregation was dependent upon receptor activation and upon arachidonic acid metabolism. Irreversible aggregation to serotonin was associated with release of 3H-serotonin and thromboxane B2 formation, indicating that a couple between the serotonergic receptor and arachidonic acid metabolism may exist.
本文介绍了一种通过提高柠檬酸钠浓度在犬富血小板血浆中获得可靠血小板产量的方法。保持安静环境或用硫喷妥钠麻醉动物有助于血小板的采集。监测了60只不同品种犬的血小板对花生四烯酸、胶原蛋白、二磷酸腺苷、肾上腺素和5-羟色胺的聚集情况。犬血小板对花生四烯酸可发生可逆或不可逆聚集。花生四烯酸不可逆聚集血小板的百分比因所检测犬的品种而异，范围从0%到100%。花生四烯酸不可逆聚集血小板在较低浓度的胶原蛋白作用下也会发生不可逆聚集，并且对二磷酸腺苷和5-羟色胺表现出双相不可逆聚集。5-羟色胺诱导的不可逆聚集依赖于受体激活和花生四烯酸代谢。对5-羟色胺的不可逆聚集与3H-5-羟色胺的释放和血栓素B2的形成有关，表明5-羟色胺能受体与花生四烯酸代谢之间可能存在偶联。
