Costa Patrícia Gomes, Dal Pizzol Juliana Lemos, Zebral Yuri Dornelles, Bianchini Adalto
Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Avenida Itália km 8, Campus Carreiros, 96.203-900, Rio Grande, RS, Brazil.
MethodsX. 2024 Aug 8;13:102896. doi: 10.1016/j.mex.2024.102896. eCollection 2024 Dec.
We searched for an extraction method that would allow a precise quantification of metal(loid)s in milligram-size samples using high-resolution graphite furnace atomic absorption spectrometry (HR-GFAAS). We digested biological (DORM-4, DOLT-5 and TORT-3) and sediment (MESS-4) certified reference materials (CRMs) using nitric acid in a drying oven, aqua regia in a drying oven, or nitric acid in a microwave. In addition, we digested MESS-4 using a mixture of nitric and hydrofluoric acids in a drying oven. We also evaluated the effect of sample size (100 and 200 mg) on the extraction efficiency. Nitric acid extraction in a drying oven yielded the greatest recovery rates for all metal(loid)s in all tested CRMs (80.0 %-100.0 %) compared with the other extraction methods tested (67.3 %-99.2 %). In most cases, the sample size did not have a significant effect on the extraction efficiency. Therefore, we conclude that nitric acid digestion in a drying oven is a reliable extraction method for milligram-size samples to quantify metal(loid)s with HR-GFAAS. This validated method could provide substantial benefits to environmental quality monitoring programs by significantly reducing the time and costs required for sample collection, storage, transport and preparation, as well as the amount of hazardous chemicals used during sample extraction and analysis. •Sample digestion with nitric acid in a drying oven yielded the greatest recovery rates of metal(loid)s from biological and sediment certified reference materials.•The recovery rates of metal(loid)s from biological and sediment certified reference materials using nitric acid digestion in a drying oven ranged from 73 % to 100 %.•Digestion with nitric acid in a drying oven is a simple and reliable method to extract small size environmental samples for metal(loid)s quantification by high-resolution graphite furnace atomic absorption spectrometry.
我们寻找了一种萃取方法，该方法能够使用高分辨率石墨炉原子吸收光谱法（HR-GFAAS）对毫克级样品中的金属（类金属）进行精确量化。我们使用烘箱中的硝酸、烘箱中的王水或微波中的硝酸对生物（DORM-4、DOLT-5和TORT-3）及沉积物（MESS-4）认证参考物质（CRM）进行消解。此外，我们还使用烘箱中的硝酸和氢氟酸混合物对MESS-4进行消解。我们还评估了样品量（100毫克和200毫克）对萃取效率的影响。与其他测试的萃取方法（67.3%-99.2%）相比，烘箱中硝酸萃取法在所有测试的CRM中对所有金属（类金属）的回收率最高（80.0%-100.0%）。在大多数情况下，样品量对萃取效率没有显著影响。因此，我们得出结论，烘箱中硝酸消解是一种用于毫克级样品的可靠萃取方法，可通过HR-GFAAS对金属（类金属）进行量化。这种经过验证的方法可为环境质量监测计划带来诸多益处，可显著减少样品采集、储存、运输和制备所需的时间和成本，以及样品萃取和分析过程中使用的有害化学物质的量。•在烘箱中用硝酸进行样品消解，从生物和沉积物认证参考物质中获得的金属（类金属）回收率最高。•在烘箱中用硝酸消解生物和沉积物认证参考物质，金属（类金属）的回收率在73%至100%之间。•在烘箱中用硝酸消解是一种简单可靠的方法，用于提取小尺寸环境样品，以通过高分辨率石墨炉原子吸收光谱法对金属（类金属）进行量化。
