Roberts N A, Robinson P A
Br J Rheumatol. 1985 May;24(2):128-36. doi: 10.1093/rheumatology/24.2.128.
An in vitro assay is described which simultaneously tests for the superoxide dismutase-like activity of low-molecular-weight copper chelates and their stability in the presence of excess EDTA. The EDTA is introduced to simulate the competitive endogenous ligands a copper-chelating drug will encounter in plasma and other body fluids. Under the conditions of the assay, antirheumatic drugs of the D-penicillamine type are clearly distinguished both from nonsteroidal anti-inflammatories and from some drugs which chelate copper but lack antirheumatic activity.
本文描述了一种体外测定方法，该方法可同时检测低分子量铜螯合物的超氧化物歧化酶样活性及其在过量乙二胺四乙酸（EDTA）存在下的稳定性。引入EDTA是为了模拟铜螯合药物在血浆和其他体液中会遇到的竞争性内源性配体。在该测定条件下，D-青霉胺类抗风湿药物与非甾体抗炎药以及一些能螯合铜但缺乏抗风湿活性的药物能明显区分开来。
