Institute of Nanotechnology, National Research Council (CNR-NANOTEC), c/o Campus Ecotekne, via Monteroni, 73100, Lecce, Italy.
Department of Mathematics and Physics ''Ennio De Giorgi", University of Salento, c/o Campus Ecotekne, via Monteroni, 73100, Lecce, Italy.
J Mater Chem B. 2024 Oct 23;12(41):10573-10583. doi: 10.1039/d4tb01047g.
Potassium cations play many important roles in living organisms, especially in electro-physiology, since they are involved in neurotransmission and muscle contractions. We report the synthesis of a ratiometric fluorescent microsensor for potassium (K) detection, based on the fluorescent probe ION potassium green 4. Potassium-sensitive fluorescent microparticles were obtained by using silica as the core material. We obtained silica-based microsensors with sizes in the micrometer range, spherical shapes, good monodispersity, optimal selectivity and a sensitivity range of 0 to 40 mM. The microsensors also proved to be non-toxic in cell cultures and suitable for fluorescence imaging, offering new possibilities for non-invasive optical detection, quantification and monitoring of K variations in cell culture systems.
钾阳离子在生物体中扮演着许多重要的角色，特别是在电生理学方面，因为它们参与神经递质传递和肌肉收缩。我们报告了一种基于荧光探针 ION potassium green 4 的钾（K）比率荧光微传感器的合成。使用二氧化硅作为核心材料，获得了对钾敏感的荧光微粒子。我们获得了尺寸在微米范围内、具有球形形状、良好的单分散性、最佳选择性和 0 至 40mM 检测范围的基于二氧化硅的微传感器。这些微传感器在细胞培养中也被证明是无毒的，适用于荧光成像，为细胞培养系统中 K 变化的非侵入性光学检测、定量和监测提供了新的可能性。
