Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.
Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.
Talanta. 2017 May 15;167:550-556. doi: 10.1016/j.talanta.2017.02.064. Epub 2017 Feb 27.
The monitoring of K is very important and emergency because of their unique relationship in various disease diagnosis and treatment. G-quadruplex analogue is a classical recognition unit for K detection and has been widely applied in K relevant research. Common fluorescent dyes were employed for design of G-quadruplex structure-based K probes which suffered from the aggregation-caused quenching effect, and possibly limited the biological applications in living systems. Herein, we report an aggregation-induced emission (AIE) effect-based fluorescent probe for cellular K analysis and imaging. Benefitting from the K triggered AIE phenomenon, the designed TPE derivative modified guanine (G)-rich oligonucleotide fluorescent probe (TPE-oligonucleotide probe) exhibits high sensitivity (∼10-fold higher than most reported G-quadruplex-based probes) with extended photostability which facilitates the prolonged fluorescence observations of K in living cells. On the basis of these advantages, the TPE-oligonucleotide probe serves as a promising candidate for the functional study and analysis of K.
由于 K 在各种疾病诊断和治疗中的独特关系，对其进行监测非常重要且紧急。G-四链体类似物是 K 检测的经典识别单元，已广泛应用于 K 相关研究中。常见的荧光染料被用于设计基于 G-四链体结构的 K 探针，但这些探针受到聚集诱导猝灭效应的影响，可能限制了它们在活细胞系统中的生物应用。在此，我们报告了一种基于聚集诱导发光（AIE）效应的用于细胞内 K 分析和成像的荧光探针。得益于 K 触发的 AIE 现象，设计的 TPE 衍生物修饰的富含鸟嘌呤（G）的寡核苷酸荧光探针（TPE-寡核苷酸探针）表现出高灵敏度（比大多数报道的基于 G-四链体的探针高约 10 倍）和延长的光稳定性，这有利于在活细胞中对 K 进行长时间荧光观察。基于这些优势，TPE-寡核苷酸探针可作为研究和分析 K 的功能的有前途的候选物。
