Department of Microbiology and Plant Pathology, University of California, Riverside, CA, USA.
Department of Statistics, University of California, Riverside, CA, USA.
J Insect Sci. 2024 Jul 1;24(4). doi: 10.1093/jisesa/ieae084.
Bemisia tabaci New World (NW) (Gennadius) (Hemiptera: Aleyrodidae), a whitefly in the B. tabaci species complex, is polyphagous on many plant species. Yet, it has been displaced, albeit not entirely, by other whitefly species. Potential causes could include issues with adaptation, feeding, and the colonization of new-hosts; however, insights that would help clarify these possibilities are lacking. Here, we sought to address these gaps by performing electropenetrography (EPG) recordings of NW whiteflies, designated "Napus" and "Rapa," reared on 2 colony hosts, Brassica napus and B. rapa, respectively. Analysis of 17 probing and pathway (pw) phase-related EPG variables revealed that the whiteflies exhibited unique probing behaviors on their respective colony hosts, with some deterrence being encountered on B. rapa. Upon switching to B. rapa and B. napus, the probing patterns of Napus and Rapa whiteflies, respectively, adapted quickly to these new-hosts to resemble that of whiteflies feeding on their colony hosts. Results for 3 of the EPG variables suggested that B. rapa's deterrence against Napus whitefly was significant prior to the phloem phase. This also suggested that adaptation by Rapa whitefly improved its pw probing on B. rapa. Based on analysis of 24 phloem phase-related EPG variables, Napus and Rapa whiteflies performed equally well once they entered phloem phase and exhibited comparable phloem acceptability on both the colony- and new-hosts. These findings demonstrate that NW whiteflies reared on a colony host are highly adaptable to feeding on a new host despite encountering some deterrence during the nonphloem phases in B. rapa plant.
烟粉虱新世界（NW）（Gennadius）（半翅目：粉虱科），是烟粉虱种复合体中的一种粉虱，对许多植物物种具有多食性。然而，它已经被其他粉虱物种所取代，尽管不是完全取代。潜在的原因可能包括适应、取食和新宿主定殖方面的问题；然而，缺乏有助于澄清这些可能性的见解。在这里，我们通过对分别在两个种群宿主甘蓝型油菜和白菜上饲养的 NW 粉虱“Napus”和“Rapa”进行电穿孔记录（EPG）来解决这些差距。对 17 个探测和途径（pw）相关 EPG 变量的分析表明，粉虱在各自的种群宿主上表现出独特的探测行为，在白菜上受到一些抑制。当它们分别切换到白菜和甘蓝型油菜时，Napus 和 Rapa 粉虱的探测模式迅速适应这些新宿主，类似于在其种群宿主上取食的粉虱。EPG 变量的 3 个结果表明，白菜对 Napus 粉虱的抑制作用在韧皮部相之前是显著的。这也表明，Rapa 粉虱的适应能力提高了其在白菜上的 pw 探测。基于对 24 个韧皮部相关 EPG 变量的分析，一旦进入韧皮部相，Napus 和 Rapa 粉虱都表现良好，并且在种群宿主和新宿主上表现出相似的韧皮部可接受性。这些发现表明，尽管在白菜植株的非韧皮部相期间遇到一些抑制，但在种群宿主上饲养的 NW 粉虱对新宿主的取食具有很强的适应性。
