Lu Shaohua, Li Jingjing, Wang Xueli, Song Danyang, Bai Rune, Shi Yan, Gu Qinsheng, Kuo Yen-Wen, Falk Bryce W, Yan Fengming
College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.
Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 410100, China.
Viruses. 2017 Jan 13;9(1):4. doi: 10.3390/v9010004.
It is known that plant viruses can change the performance of their vectors. However, there have been no reports on whether or how a semipersistent plant virus manipulates the feeding behaviors of its whitefly vectors. Cucurbit chlorotic yellows virus (CCYV) (genus , family ) is an emergent plant virus in many Asian countries and is transmitted specifically by B and Q biotypes of tobacco whitefly, (Gennadius), in a semipersistent manner. In the present study, we used electrical penetration graph (EPG) technique to investigate the effect of CCYV on the feeding behaviors of . The results showed that CCYV altered feeding behaviors of both biotypes and sexes of with different degrees. CCYV had stronger effects on feeding behaviors of Q biotype than those of B biotype, by increasing duration of phloem salivation and sap ingestion, and could differentially manipulate feeding behaviors of males and females in both biotype whiteflies, with more phloem ingestion in Q biotype males and more non-phloem probing in B biotype males than their respective females. With regard to feeding behaviors related to virus transmission, these results indicated that, when carrying CCYV, Q biotype plays more roles than B biotype, and males make greater contribution than females.
已知植物病毒可改变其传毒介体的行为。然而，关于半持久性植物病毒是否以及如何操控其粉虱传毒介体的取食行为，尚无相关报道。葫芦褪绿黄化病毒（CCYV）（属，科）是许多亚洲国家出现的一种植物病毒，由烟粉虱（Gennadius）的B型和Q型生物型以半持久性方式专性传播。在本研究中，我们使用电穿透图（EPG）技术来研究CCYV对烟粉虱取食行为的影响。结果表明，CCYV不同程度地改变了烟粉虱两种生物型和两性的取食行为。CCYV对Q型生物型取食行为的影响比对B型生物型更强，它增加了韧皮部唾液分泌和汁液摄取的持续时间，并且能够不同程度地操控两种生物型粉虱中雄性和雌性的取食行为，Q型生物型雄性比其雌性摄取更多的韧皮部汁液，B型生物型雄性比其雌性进行更多的非韧皮部探测。关于与病毒传播相关的取食行为，这些结果表明，携带CCYV时，Q型生物型比B型生物型发挥的作用更大，且雄性比雌性贡献更大。
