Department of Clinical Laboratory Medicine, The People's Hospital of Chongqing Liangjiang New Area, No. 199 Ren Xing Road, Yubei, Chongqing, 401121, PR China.
Department of Clinical Laboratory Medicine, Southwest Hospital, Army Medical University, Chongqing, 400038, PR China.
Anal Methods. 2024 Sep 26;16(37):6443-6450. doi: 10.1039/d4ay01410c.
Accurate detection of tumor biomarkers in blood is crucial for diagnosing and treating tumor disease. In this study, a metal enzyme-linked immunosorbent assay (MeLISA) was fabricated for the ultrasensitive and naked-eye detection of tumor biomarker alpha-fetoprotein (AFP) in clinical serum samples. Herein, novel copper metal-organic frameworks and gold platinum nanoparticle composites (Cu-MOFs@AuPtNPs) were synthesized for the first time by an method, which showed an enormous specific surface area and excellent peroxidase (POx) mimicking properties. Cu-MOFs@AuPtNPs linked with antibodies targeting AFP served as a signal nanoprobe to amplify the detection signal. Additionally, the specificity of MeLISA was significantly enhanced through a conventional antigen-antibody reaction and efficient blocking of non-specific sites with BSA. Under optimal conditions, the sandwich-type MeLISA exhibited a wide range from 0.001 to 1000 ng mL ( = 0.997) and a low detection limit of 0.86 pg mL (S/N = 3) with acceptable stability, selectivity, and reproducibility. It is noteworthy that the suggested MeLISA performed exceptionally well in detecting clinical serum samples, which were visible to the naked eye and did not require complex platforms. To sum up, the innovative MeLISA based on Cu-MOFs@AuPtNPs provides an alternative method for early cancer diagnosis, particularly in economically backward areas where simple diagnostic apparatus is extremely desirable.
准确检测血液中的肿瘤标志物对于肿瘤疾病的诊断和治疗至关重要。在本研究中，构建了一种金属酶联免疫吸附测定（MeLISA），用于临床血清样本中肿瘤标志物甲胎蛋白（AFP）的超灵敏和肉眼检测。在此，首次通过一种简便的方法合成了新颖的铜金属有机骨架和金铂纳米粒子复合材料（Cu-MOFs@AuPtNPs），其具有巨大的比表面积和优异的过氧化物酶（POx）模拟性能。与针对 AFP 的抗体偶联的 Cu-MOFs@AuPtNPs 用作信号探针以放大检测信号。此外，通过常规的抗原-抗体反应以及用 BSA 有效封闭非特异性位点，大大提高了 MeLISA 的特异性。在最佳条件下，夹心型 MeLISA 的检测范围为 0.001 至 1000 ng mL（ = 0.997），检测限低至 0.86 pg mL（S/N = 3），具有良好的稳定性、选择性和重现性。值得注意的是，所提出的 MeLISA 在检测临床血清样本方面表现出色，肉眼可见，且不需要复杂的平台。总之，基于 Cu-MOFs@AuPtNPs 的创新型 MeLISA 为早期癌症诊断提供了一种替代方法，特别是在经济落后地区，非常需要简单的诊断仪器。
