State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, P. R. China.
School of Electrical Engineering, University of South China, Hengyang 421001, P. R. China.
Nano Lett. 2024 Sep 18;24(37):11590-11598. doi: 10.1021/acs.nanolett.4c03099. Epub 2024 Sep 3.
As a nonenzymatic DNA signal amplification technique, localized hybridization chain reaction (LHCR) was designed to improve the limitations in response speed and low sensitivity of conventional free diffusional HCR (hybridization chain reaction). However, it is still confronted with the challenges of complicated DNA scaffolds with low loading capacity and a time-consuming process of diffusion. Herein, we introduced modular assembly of a DNA minimal scaffold for coassembly of DNA hairpins for amplified fluorescence imaging of mRNA in situ. DNA hairpins were spatially bound to two Y-shaped modules to form H-shaped DNA modules, and then multiple H-shaped DNA modules can further assemble into an H-module-based hairpin scaffold (HHS). Benefiting from highly spatial localization and high loading capacity, the HHS system showed higher sensitivity and faster speed. It has also been proven to work perfectly in vitro and in vivo, which could provide a promising bioanalysis system for low abundance biomolecule detection.
作为一种非酶 DNA 信号放大技术，局部杂交链式反应 (LHCR) 被设计用来提高传统自由扩散 HCR（杂交链式反应）在响应速度和灵敏度方面的局限性。然而，它仍然面临着具有低载量的复杂 DNA 支架和扩散过程耗时的挑战。在这里，我们引入了 DNA 最小支架的模块化组装，用于 DNA 发夹的共组装，以进行原位 mRNA 的放大荧光成像。DNA 发夹被空间束缚在两个 Y 形模块上，形成 H 形 DNA 模块，然后多个 H 形 DNA 模块可以进一步组装成基于 H 模块的发夹支架 (HHS)。受益于高度的空间定位和高载量，HHS 系统表现出更高的灵敏度和更快的速度。它已经被证明在体外和体内都能完美工作，这为低丰度生物分子的检测提供了一个很有前途的生物分析系统。
