Willis G L, Wreford N G, Smith G C
Brain Res. 1985 May 20;334(2):344-7. doi: 10.1016/0006-8993(85)90229-x.
Microspectrofluorometry was employed to identify the spectral characteristics of the formaldehyde-induced fluorophore derived from amine accumulation proximal to the site of 6-hydroxydopamine injection or radiofrequency lesions in the lateral hypothalamus of Sprague-Dawley rats. The excitation and emission spectra of the accumulation were consistent with that of catecholamines in models subjected to similar formaldehyde treatment. These results indicate that while biochemical assay technique may not permit the quantification of amine accumulations, these areas do in fact represent degeneration associated increases in catecholamines which may be of functional significance.
采用显微分光荧光测定法来鉴定源自6-羟基多巴胺注射部位或Sprague-Dawley大鼠下丘脑外侧射频损伤部位附近胺类积累的甲醛诱导荧光团的光谱特征。在接受类似甲醛处理的模型中，这种积累的激发光谱和发射光谱与儿茶酚胺的光谱一致。这些结果表明，虽然生化检测技术可能无法对胺类积累进行定量，但这些区域实际上确实代表了与儿茶酚胺增加相关的变性，这可能具有功能意义。
