Smith G C, Courtney P G, Wreford N G, Walker M M
Brain Res. 1982 Feb 18;234(1):101-10. doi: 10.1016/0006-8993(82)90475-9.
6-Hydroxydopamine (6-OHDA) given i.v. in a dose of 150 mg/kg to adult male Sprague-Dawley rats produces at 24 h a complete depletion of median eminence (ME) and neurointermediate lobe (NIL) catecholamines (CA), as judged by Falck-Hillarp fluorescence histochemistry, leaving the remainder of the hypothalamus substantially unaltered. Restoration of a normal fluorescence histochemical appearance took place over 35 days, apparently due to regeneration of CA-containing terminals. Administration of desipramine prior to 6-OHDA injection modified its effect; depletion was largely confined to the midline region of the external layer of the ME and to the NIL, suggesting that these regions contain dopaminergic terminals. Microspectrofluorometric methods were used in an attempt to verify this conclusion. It is suggested that this technique provides a model for establishing the role of ME and NIL CA-containing structures in control of pituitary gland function.
给成年雄性斯普拉格-道利大鼠静脉注射剂量为150毫克/千克的6-羟基多巴胺（6-OHDA），24小时后，通过福尔克-希拉尔普荧光组织化学法判断，正中隆起（ME）和神经中间叶（NIL）的儿茶酚胺（CA）完全耗竭，而下丘脑其余部分基本未改变。正常的荧光组织化学外观在35天内恢复，这显然是由于含CA的终末再生所致。在注射6-OHDA之前给予地昔帕明可改变其作用；耗竭主要局限于ME外层的中线区域和NIL，这表明这些区域含有多巴胺能终末。使用显微分光荧光测定法试图验证这一结论。有人认为，该技术为确定ME和含NIL CA结构在垂体功能控制中的作用提供了一个模型。
