Kulis Iu Iu, Kadziauskene K V, Vidzhiunaĭte R A
Mikrobiologiia. 1979 Jul-Aug;48(4):639-44.
Lactate oxidation by freeze-dried cells of Hansenula anomala was studied in the presence of exogenous electron acceptors, potassium ferricyanide (PF) and phenazine metosulfate (PMS). The product of oxidation is pyruvate. The apparent Michaelis constants for lactate, PF and PMS are 1.2, 0.6 and 0.05, respectively. The pH optimum of lactate oxidation is 8.0. The freeze-dried cells of the yeast are inactivated by 5 mM bromopyruvate by 50% within 1.5 min. Treatment of the intact cells with ultrasound activates them. The respiratory function of the cells is characterized with a KM(app.) of 0.05 mM in the presence of lactate. PF has no effect on respiration. A scheme is proposed for lactate oxidation by the cells with damaged membranes whose amount after lyophilization is 10--20%.
在存在外源电子受体铁氰化钾（PF）和吩嗪硫酸甲酯（PMS）的情况下，研究了异常汉逊酵母冻干细胞对乳酸的氧化作用。氧化产物是丙酮酸。乳酸、PF和PMS的表观米氏常数分别为1.2、0.6和0.05。乳酸氧化的最适pH为8.0。5 mM溴丙酮酸在1.5分钟内可使酵母冻干细胞失活50%。用超声处理完整细胞可激活它们。在存在乳酸的情况下，细胞的呼吸功能以表观米氏常数KM(app.)为0.05 mM来表征。PF对呼吸无影响。提出了细胞膜受损的细胞对乳酸氧化的方案，冻干后细胞膜受损的细胞数量为10%-20%。
