• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Olink 蛋白质组学在绝经后骨质疏松症早期诊断生物标志物鉴定中的应用。

Olink Proteomics for the Identification of Biomarkers for Early Diagnosis of Postmenopausal Osteoporosis.

机构信息

Beijing Jishuitan Hospital, Capital Medical University, Xicheng District, Beijing 100035, China.

Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing 100053, China.

出版信息

J Proteome Res. 2024 Oct 4;23(10):4567-4578. doi: 10.1021/acs.jproteome.4c00470. Epub 2024 Sep 3.

DOI:10.1021/acs.jproteome.4c00470
PMID:39226440
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11460326/
Abstract

This investigation aims to employ Olink proteomics in analyzing the distinct serum proteins associated with postmenopausal osteoporosis (PMOP) and identifying prognostic markers for early detection of PMOP via molecular mechanism research on postmenopausal osteoporosis. Postmenopausal women admitted to Beijing Jishuitan Hospital were randomly selected and categorized into three groups based on their dual-energy X-ray absorptiometry (DXA) T-scores: osteoporosis group ( 24), osteopenia group ( 20), and normal bone mass group ( 16). Serum samples from all participants were collected for clinical and bone metabolism marker measurements. Olink proteomics was utilized to identify differentially expressed proteins (DEPs) that are highly associated with postmenopausal osteoporosis. The functional analysis of DEPs was performed using Gene Ontology and Kyto Encyclopedia Genes and Genomes (KEGG). The biological characteristics of these proteins and their correlation with PMOP were subsequently analyzed. ROC curve analysis was performed to identify potential biomarkers with the highest diagnostic accuracy for early stage PMOP. Through Olink proteomics, we identified five DEPs highly associated with PMOP, including two upregulated and three downregulated proteins. TWEAK and CDCP1 markers exhibited the highest area under the curve (0.8188 and 0.8031, respectively). TWEAK and CDCP1 have the potential to serve as biomarkers for early prediction of postmenopausal osteoporosis.

摘要

本研究旨在通过对绝经后骨质疏松症的分子机制研究，运用 Olink 蛋白质组学分析与绝经后骨质疏松症相关的不同血清蛋白，并鉴定用于早期检测绝经后骨质疏松症的预后标志物。随机选取北京积水潭医院收治的绝经后女性，根据双能 X 线吸收仪（DXA）T 评分将其分为三组：骨质疏松组（24 例）、骨量减少组（20 例）和正常骨量组（16 例）。采集所有参与者的血清样本，进行临床和骨代谢标志物测量。采用 Olink 蛋白质组学技术鉴定与绝经后骨质疏松症高度相关的差异表达蛋白（DEPs）。通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）对 DEPs 进行功能分析。分析这些蛋白的生物学特性及其与 PMOP 的相关性。通过 ROC 曲线分析，鉴定具有最高诊断准确性的早期 PMOP 潜在生物标志物。通过 Olink 蛋白质组学，我们鉴定出与 PMOP 高度相关的五个 DEPs，包括两个上调和三个下调蛋白。TWEAK 和 CDCP1 标志物的曲线下面积最高（分别为 0.8188 和 0.8031）。TWEAK 和 CDCP1 有望成为绝经后骨质疏松症早期预测的生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/c5f3119381d3/pr4c00470_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/b60338285953/pr4c00470_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/7ce608200da8/pr4c00470_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/faddd7cec4e2/pr4c00470_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/a61bc8f374a1/pr4c00470_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/34c79e0f2a2c/pr4c00470_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/272b1d01abfd/pr4c00470_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/274d181f8601/pr4c00470_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/c5f3119381d3/pr4c00470_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/b60338285953/pr4c00470_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/7ce608200da8/pr4c00470_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/faddd7cec4e2/pr4c00470_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/a61bc8f374a1/pr4c00470_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/34c79e0f2a2c/pr4c00470_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/272b1d01abfd/pr4c00470_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/274d181f8601/pr4c00470_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77e3/11460326/c5f3119381d3/pr4c00470_0008.jpg

相似文献

1
Olink Proteomics for the Identification of Biomarkers for Early Diagnosis of Postmenopausal Osteoporosis.Olink 蛋白质组学在绝经后骨质疏松症早期诊断生物标志物鉴定中的应用。
J Proteome Res. 2024 Oct 4;23(10):4567-4578. doi: 10.1021/acs.jproteome.4c00470. Epub 2024 Sep 3.
2
Correlation of serum pentraxin 3 expression with disease severity and its prognostic value in postmenopausal osteoporosis.血清五聚体蛋白3表达与绝经后骨质疏松症疾病严重程度的相关性及其预后价值
BMC Musculoskelet Disord. 2025 Sep 1;26(1):834. doi: 10.1186/s12891-025-09091-z.
3
Biomarkers and therapeutic targets in early Alzheimer's disease: an Olink proteomics study.早期阿尔茨海默病中的生物标志物与治疗靶点：一项Olink蛋白质组学研究
Front Neurol. 2025 Jul 17;16:1615152. doi: 10.3389/fneur.2025.1615152. eCollection 2025.
4
Plasma serotonin precursors and metabolites as diagnostic and therapeutic biomarkers for osteoporosis in postmenopausal women.血浆血清素前体和代谢产物作为绝经后女性骨质疏松症的诊断和治疗生物标志物。
Biomol Biomed. 2025 Jun 11. doi: 10.17305/bb.2025.11513.
5
Identification and validation of , , and as potential diagnostic markers of postmenopausal osteoporosis.鉴定并验证[具体内容未给出，用逗号隔开的部分]作为绝经后骨质疏松症潜在诊断标志物。
Biomol Biomed. 2025 Aug 11. doi: 10.17305/bb.2025.12019.
6
Value of serum uric acid in a risk prediction model for postmenopausal osteoporosis.血清尿酸在绝经后骨质疏松症风险预测模型中的价值
Arch Gynecol Obstet. 2025 Aug 9. doi: 10.1007/s00404-025-08119-y.
7
Identification of osteoporosis ferroptosis-related markers and potential therapeutic compounds based on bioinformatics methods and molecular docking technology.基于生物信息学方法和分子对接技术鉴定骨质疏松症铁死亡相关标志物和潜在治疗化合物。
BMC Med Genomics. 2024 Apr 22;17(1):99. doi: 10.1186/s12920-024-01872-0.
8
Single-cell proteomics analysis of human oocytes during GV-to-MI transition.人卵母细胞从生发泡期到第一次减数分裂中期转变过程中的单细胞蛋白质组学分析
Hum Reprod. 2025 May 13. doi: 10.1093/humrep/deaf086.
9
LncRNA SDCBP2-AS1 is a putative biomarker for postmenopausal osteoporosis and promotes osteogenic differentiation of BMSCs by regulating miR-361-3p.长链非编码RNA SDCBP2-AS1是绝经后骨质疏松症的一种潜在生物标志物，并通过调控miR-361-3p促进骨髓间充质干细胞的成骨分化。
Hereditas. 2025 Jul 10;162(1):124. doi: 10.1186/s41065-025-00494-5.
10
Association between lncRNA H19 and osteoporosis in postmenopausal patients with type 2 diabetes mellitus.lncRNA H19与绝经后2型糖尿病患者骨质疏松症之间的关联
J Orthop Surg Res. 2025 Aug 7;20(1):737. doi: 10.1186/s13018-025-06153-9.

引用本文的文献

1
Bridging Genomic Research Disparities in Osteoporosis GWAS: Insights for Diverse Populations.弥合骨质疏松症全基因组关联研究中的基因组研究差距：对不同人群的见解
Curr Osteoporos Rep. 2025 May 24;23(1):24. doi: 10.1007/s11914-025-00917-2.

本文引用的文献

1
Metastatic prostate cancer-derived extracellular vesicles facilitate osteoclastogenesis by transferring the CDCP1 protein.转移性前列腺癌衍生的细胞外囊泡通过转移 CDCP1 蛋白促进破骨细胞生成。
J Extracell Vesicles. 2023 Mar;12(3):e12312. doi: 10.1002/jev2.12312.
2
CUB Domain-Containing Protein 1 (CDCP1) is a rational target for the development of imaging tracers and antibody-drug conjugates for cancer detection and therapy.CUB 结构域包含蛋白 1（CDCP1）是开发成像示踪剂和用于癌症检测和治疗的抗体药物偶联物的合理靶标。
Theranostics. 2022 Oct 3;12(16):6915-6930. doi: 10.7150/thno.78171. eCollection 2022.
3
The Rising Era of "Immunoporosis": Role of Immune System in the Pathophysiology of Osteoporosis.
“免疫性骨质疏松症”的崛起时代：免疫系统在骨质疏松症病理生理学中的作用
J Inflamm Res. 2022 Mar 5;15:1667-1698. doi: 10.2147/JIR.S351918. eCollection 2022.
4
Prevalence of Osteoporosis and Fracture in China: The China Osteoporosis Prevalence Study.中国骨质疏松症患病率及骨折患病情况：中国骨质疏松症流行病学调查。
JAMA Netw Open. 2021 Aug 2;4(8):e2121106. doi: 10.1001/jamanetworkopen.2021.21106.
5
Pathophysiology of moderate to severe plaque psoriasis: anti-IL-17 towards disease modification.中重度斑块型银屑病的病理生理学：抗 IL-17 治疗疾病的改变。
Drugs Today (Barc). 2021 May;57(5):347-357. doi: 10.1358/dot.2021.57.5.3266244.
6
Efficacy and safety of secukinumab in Chinese patients with moderate-to-severe plaque psoriasis: a real-life cohort study.司库奇尤单抗治疗中国中重度斑块状银屑病患者的疗效和安全性：一项真实世界队列研究。
Chin Med J (Engl). 2021 May 4;134(11):1324-1328. doi: 10.1097/CM9.0000000000001510.
7
Lactobacillus rhamnosus attenuates bone loss and maintains bone health by skewing Treg-Th17 cell balance in Ovx mice.罗伊氏乳杆菌通过调整去卵巢小鼠中 Treg-Th17 细胞平衡来减轻骨丢失和维持骨骼健康。
Sci Rep. 2021 Jan 19;11(1):1807. doi: 10.1038/s41598-020-80536-2.
8
Treatment-related changes in bone mineral density as a surrogate biomarker for fracture risk reduction: meta-regression analyses of individual patient data from multiple randomised controlled trials.作为降低骨折风险的替代生物标志物的骨密度治疗相关变化：来自多个随机对照试验的个体患者数据的荟萃回归分析。
Lancet Diabetes Endocrinol. 2020 Aug;8(8):672-682. doi: 10.1016/S2213-8587(20)30159-5.
9
A road map for understanding molecular and genetic determinants of osteoporosis.骨质疏松症分子和遗传决定因素的理解路线图。
Nat Rev Endocrinol. 2020 Feb;16(2):91-103. doi: 10.1038/s41574-019-0282-7. Epub 2019 Dec 2.
10
Psoriasis Pathogenesis and Treatment.银屑病发病机制与治疗。
Int J Mol Sci. 2019 Mar 23;20(6):1475. doi: 10.3390/ijms20061475.