lncRNA H19与绝经后2型糖尿病患者骨质疏松症之间的关联

Association between lncRNA H19 and osteoporosis in postmenopausal patients with type 2 diabetes mellitus.

作者信息

Yang Teng, Feng Xiuli, Wang Na, Zhang Tongyu, Zheng Zhichen, Wang Guofeng

机构信息

Department of Endocrinology, Jinzhou Medical University Lianyungang First People's Hospital Graduate Training Base, Lianyungang, 222000, China.

Department of Endocrinology, Lianyungang Clinical College of Nanjing Medical University, Lianyungang, 222000, China.

出版信息

J Orthop Surg Res. 2025 Aug 7;20(1):737. doi: 10.1186/s13018-025-06153-9.

DOI:10.1186/s13018-025-06153-9

PMID:40775721

Abstract

BACKGROUND

This study aimed to assess the expression level of long non-coding RNA H19 (lncRNA H19) in peripheral blood mononuclear cells (PBMCs) of postmenopausal patients with type 2 diabetes mellitus (T2DM) with comorbid osteoporosis (T2DM-OP), and to evaluate its diagnostic value and its role in the pathogenesis of T2DM-OP.

METHODS

A total of 150 postmenopausal patients with T2DM were recruited from the First People's Hospital of Lianyungang City, China. Based on the bone mineral density (BMD) results measured by dual-energy X-ray absorptiometry (DXA), the patients were stratified into the T2DM-OP group (n = 108) and the T2DM group (n = 42). Clinical data and laboratory parameters were collected, and quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to quantitatively determine the expression of lncRNA H19 in PBMCs.

RESULTS

When compared with the T2DM group, the expression level of lncRNA H19 in PBMCs of T2DM-OP patients was significantly lower (P < 0.001). The results of bivariate analysis revealed that lncRNA H19 was positively correlated with albumin (ALB, R = 0.311) and BMD (R = 0.696), while negatively correlated with age (R = -0.514), glycated hemoglobin A1c (HbA1c, R = -0.272), fasting blood glucose (FBG, R = -0.275), and transforming growth factor-β1 (TGF-β1, R = -0.392). All of these correlations were statistically significant (R < 0.001). Binary logistic regression analysis identified age, body mass index (BMI), HbA1c, TGF-β1, and lncRNA H19 as independent risk factors for T2DM-OP. Additionally, multiple linear regression analysis demonstrated that age, HbA1c, FBG, TGF-β1, and BMD were key determinants of the expression of lncRNA H19. Receiver operating characteristic (ROC) curve analysis indicated that lncRNA H19 had high diagnostic accuracy for postmenopausal T2DM-OP, with an area under the curve (AUC) of 0.833.

CONCLUSIONS

The expression level of lncRNA H19 in PBMCs of postmenopausal T2DM-OP patients was significantly reduced. This reduction could contribute to the pathogenesis of T2DM-OP through the regulation of modulating glucose metabolism and the lncRNA H19/TGF-β1 signaling pathway. Moreover, its expression level may serve as a highly promising non-invasive biomarker with high diagnostic accuracy for the early detection of postmenopausal T2DM-OP.

TRIAL REGISTRATION

Retrospectively registered (ChiCTR2300074807).

摘要

背景

本研究旨在评估绝经后2型糖尿病（T2DM）合并骨质疏松症（T2DM-OP）患者外周血单个核细胞（PBMC）中长链非编码RNA H19（lncRNA H19）的表达水平，并评估其诊断价值及其在T2DM-OP发病机制中的作用。

方法

从中国连云港市第一人民医院招募了150例绝经后T2DM患者。根据双能X线吸收法（DXA）测量的骨密度（BMD）结果，将患者分为T2DM-OP组（n = 108）和T2DM组（n = 42）。收集临床资料和实验室参数，并采用定量逆转录聚合酶链反应（qRT-PCR）定量测定PBMC中lncRNA H19的表达。

结果

与T2DM组相比，T2DM-OP患者PBMC中lncRNA H19的表达水平显著降低（P < 0.001）。双变量分析结果显示，lncRNA H19与白蛋白（ALB，R = 0.311）和BMD（R = 0.696）呈正相关，而与年龄（R = -0.514）、糖化血红蛋白A1c（HbA1c，R = -0.272）、空腹血糖（FBG，R = -0.275）和转化生长因子-β1（TGF-β1，R = -0.392）呈负相关。所有这些相关性均具有统计学意义（R < 0.001）。二元逻辑回归分析确定年龄、体重指数（BMI）、HbA1c、TGF-β1和lncRNA H19为T2DM-OP的独立危险因素。此外，多元线性回归分析表明年龄、HbA1c、FBG、TGF-β1和BMD是lncRNA H19表达的关键决定因素。受试者工作特征（ROC）曲线分析表明，lncRNA H19对绝经后T2DM-OP具有较高的诊断准确性，曲线下面积（AUC）为0.833。