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特性玉米醇溶蛋白-丁香酚纳米纤维的细胞遗传毒性(大蒜和 MTT)揭示及其对鸡肉品质的影响。

The revealing of the Cyto-genotoxic properties (Allium and MTT) and the effect of chicken meat quality of characterized zein-eugenol nanofibers.

机构信息

Bartin University, Science Faculty, Department of Molecular Biology and Genetics/Biotechnology, 74000 Bartin, Turkey.

Ankara University, Science Faculty, Department of Physics, 06100 Ankara, Turkey; Bartin University, Bartin 74100, Turkey.

出版信息

Food Chem. 2025 Jan 1;462:141043. doi: 10.1016/j.foodchem.2024.141043. Epub 2024 Aug 30.

DOI:10.1016/j.foodchem.2024.141043
PMID:39226646
Abstract

Electrospun zein-based eugenol nanofibers (ZEnF) with diameters (148.19-631.52 nm) were fabricated. Thermal degradation was found as <15 % until 300 °C while the nanofiber diffraction pattern presented three main peaks among the 5 and 45 positions. ZEnF was not only evaluated as non-toxic to cells but also possessed anticancer characteristics revealing with the MCF-7 cell line at 800 μg/mL (reduction: 18.08 %) and 1600 μg/mL (reduction: 41.64 %). Allium tests revealed that ZEnF did not have any adverse impact on the health status (chromosomes-DNA) of exposed organisms. Following the nanofiber coating for chicken meat parts (thigh and breast), it was observed up to 1.25 log CFU/g limitation in total viable bacteria counts (p < 0.05). The sensory score (difference: 3.64 in 10 points scoring on the 6th day of the cold storage) and odor score of chicken meat samples were found to be as higher than control samples (p < 0.05).

摘要

静电纺丝制备的玉米醇溶蛋白-丁香酚纳米纤维(ZEnF)的直径为(148.19-631.52nm)。发现热降解率在 300°C 之前小于 15%,而纳米纤维衍射图谱在 5 到 45 位置之间呈现出三个主要峰值。ZEnF 不仅被评估为对细胞无毒,而且具有抗癌特性,在 MCF-7 细胞系中浓度为 800μg/mL(降低 18.08%)和 1600μg/mL(降低 41.64%)时表现出这种特性。大蒜试验表明,ZEnF 对暴露生物的健康状况(染色体-DNA)没有任何不良影响。在对鸡肉(大腿和鸡胸)部分进行纳米纤维涂层后,观察到总活菌数的限量减少了 1.25 个对数 CFU/g(p<0.05)。鸡肉样本的感官评分(在冷藏的第 6 天,10 分制的差异为 3.64)和气味评分均高于对照组(p<0.05)。

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