Department of Chemical Engineering, National Chung Hsing University, 145 Xinda Road, South District, Taichung 402, Taiwan.
Department of Chemical Engineering, National Chung Hsing University, 145 Xinda Road, South District, Taichung 402, Taiwan.
J Biosci Bioeng. 2024 Nov;138(5):445-451. doi: 10.1016/j.jbiosc.2024.08.001. Epub 2024 Sep 3.
The effect of delignification on the adsorption capacity of loofah sponge-based immobilized metal affinity chromatography adsorbents was investigated with recombinant His-tagged trehalose synthase as the model protein. Pretreatments with [EMIM][Ac] ionic liquid at 80 °C for 5 h and with sodium chlorite/acetic acid at 80 °C for 2 h were found effective for the removal of lignin, leading to a loss in biomass of 15.7% and 25.2%, respectively. Upon delignification, the metal chelating capacities of the loofah sponge-based adsorbents prepared with 5-h ionic liquid pretreatment (712 ± 82 μmole Cu(II)/g) and with 2-h sodium chlorite/acetic acid pretreatment (1012 ± 18 μmole Cu(II)/g) were 38% and 97% higher than that of the control (514 ± 55 μmole Cu(II)/g), adsorbent prepared with untreated loofah sponge, respectively. Results of protein adsorption study indicated that the Co(II)-loaded adsorbent prepared with 2-h sodium chlorite/acetic acid pretreatment exhibited the highest adsorption capacity and selectivity for the recombinant His-tagged trehalose synthase, giving a purification product with a specific activity of 7.62 U/mg protein. The predicted maximum adsorption capacity of the delignified loofah sponge-based adsorbent, 2.04 ± 0.14 mg/g, was 73% higher than that of the control.
用重组 His 标记海藻糖合酶作为模型蛋白研究了脱木质素对丝瓜络固定化金属亲和层析吸附剂吸附能力的影响。在 80°C 下用[EMIM][Ac]离子液体预处理 5 h 和在 80°C 下用亚氯酸钠/乙酸预处理 2 h 被发现可有效去除木质素，分别导致生物质损失 15.7%和 25.2%。脱木质素后，用 5 h 离子液体预处理（712 ± 82 μmole Cu(II)/g）和 2 h 亚氯酸钠/乙酸预处理制备的丝瓜络基吸附剂的金属螯合能力分别比对照（514 ± 55 μmole Cu(II)/g）和未处理丝瓜络制备的吸附剂（1012 ± 18 μmole Cu(II)/g）高 38%和 97%。蛋白质吸附研究结果表明，用 2 h 亚氯酸钠/乙酸预处理制备的 Co(II)负载吸附剂对重组 His 标记海藻糖合酶表现出最高的吸附容量和选择性，得到的纯化产物比活为 7.62 U/mg 蛋白。脱木质素丝瓜络基吸附剂的预测最大吸附容量为 2.04 ± 0.14 mg/g，比对照高 73%。
