Nasiri Leila, Vaez-Mahdavi Mohammad-Reza, Hassanpour Hossein, Ghazanfari Tooba, Kaboudanian Ardestani Sussan, Askari Nayere, Ghaffarpour Sara, Zamani Mohammad Saber
Department of Health Equity, Immunoregulation Research Center, Shahed University, Tehran, Iran.
Department of Physiology, Medical Faculty, Shahed University, Tehran, Iran.
Drug Chem Toxicol. 2025 May;48(3):668-676. doi: 10.1080/01480545.2024.2395571. Epub 2024 Sep 3.
Sulfur mustard (SM) exposure has delayed harmful effects, including premature biological aging. This study aimed to evaluate the expression of aging markers (i.e., ANRIL, P16, TBX2, and TERRA) and assess their correlation with the severity of SM exposure in the long term. The study was conducted on two volunteer groups. 1) SM-exposed group, exposed to SM once in 1987 during the war; divided into three subgroups based on the injury severity, asymptomatic (without any clinical signs), mild, and severe; 2) Non-exposed group. In the SM-exposed group, ANRIL transcript was decreased, especially in subgroups of mild and severe. TBX2 transcript was also decreased in the total SM-exposed group. This decrease was more significant in the mild and severe subgroups than in asymptomatic ones. P16 transcript was increased in the SM-exposed group, especially in the asymptomatic subgroup. The increase in TERRA transcript was also significant in all subgroups. There was a positive correlation between the TERRA transcript and the severity of injury, while this correlation was negative for the ANRIL. It is concluded that the delayed toxicity of SM may be associated with dysregulation of aging markers leading to premature cellular aging. These markers' alterations differed according to the severity of SM injury.
硫芥（SM）暴露会产生延迟性有害影响，包括生物过早衰老。本研究旨在评估衰老标志物（即ANRIL、P16、TBX2和TERRA）的表达，并长期评估它们与SM暴露严重程度的相关性。该研究在两个志愿者组中进行。1）SM暴露组，在1987年战争期间曾接触过一次SM；根据损伤严重程度分为三个亚组，无症状（无任何临床体征）、轻度和重度；2）未暴露组。在SM暴露组中，ANRIL转录本减少，尤其是在轻度和重度亚组中。TBX2转录本在整个SM暴露组中也减少。这种减少在轻度和重度亚组中比在无症状亚组中更显著。P16转录本在SM暴露组中增加，尤其是在无症状亚组中。TERRA转录本在所有亚组中的增加也很显著。TERRA转录本与损伤严重程度呈正相关，而与ANRIL呈负相关。结论是，SM的延迟毒性可能与衰老标志物的失调有关，导致细胞过早衰老。这些标志物的改变因SM损伤的严重程度而异。
