Department of Chemistry, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, 127 Youyi Road, Xi'an, 710072, China.
Mikrochim Acta. 2024 Sep 4;191(10):573. doi: 10.1007/s00604-024-06646-0.
Tannic acid (TA)-derived carbon dots (TACDs) were synthesized for the first time via a solvothermal method using TA as one of the raw materials, which may effectively inhibit amyloid fibril aggregation and disaggregate mature fibril. The fluorescent property of TACDs were modulated by adjusting the ratio of TA to o-phenylenediamine (oPD), and TACDs fabricated with the precursor ratio as 1:1 showed the best fluorescent property. Circular dichroism spectra (CD) showed that the structure of β-sheet decreased as the concentration of TACDs increased. The inhibition efficiency, as confirmed by thioflavin T (ThT) and transmission electron microscopy (TEM), is extraordinary at 98.16%, whereas disaggregation efficiency is noteworthy at 97.97%, and the disaggregated lysozyme fibrils did not reaggregate after 7 days. More critically, TACDs can also alleviate the cellular toxicity caused by Aβ fibrils and improve cell viability. This work offers a new perspective on the design of scavengers for amyloid plaques.
单宁酸（TA）衍生的碳点（TACDs）首次通过溶剂热法合成，以 TA 为原料之一，可有效抑制淀粉样纤维聚集和解聚成熟纤维。通过调节 TA 与邻苯二胺（oPD）的比例来调节 TACDs 的荧光性质，当前驱物比例为 1:1 时，TACDs 表现出最佳的荧光性质。圆二色光谱（CD）表明，随着 TACDs 浓度的增加，β-折叠结构减少。通过噻唑蓝（ThT）和透射电子显微镜（TEM）证实的抑制效率非常高，达到 98.16%，而解聚效率也非常显著，达到 97.97%，并且解聚的溶菌酶纤维在 7 天后不会再聚集。更重要的是，TACDs 还可以减轻由 Aβ 纤维引起的细胞毒性并提高细胞活力。这项工作为设计淀粉样斑块清除剂提供了新的视角。
