Adams J S, Gacad M A, Baker A J, Kheun G, Rude R K
Endocrinology. 1985 Jun;116(6):2523-7. doi: 10.1210/endo-116-6-2523.
We investigated the occurrence of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]-resistant osteomalacia in the New World primate colony of Saguinus imperator at the Los Angeles Zoo. The mean serum concentration of 1,25-(OH)2D3 was elevated 5-fold in the New World primates compared to that in their Old World counterparts. The specific internalization of 0.6 nM [3H]1,25-(OH)2D3 by cultured dermal fibroblasts from New World primates was reduced 75% compared to that by cells from Old World primates or man. The decrease in hormone uptake resulted from a decrease in the number of high affinity intracellular binding sites for 1,25-(OH)2D3 and apparently caused a 90-95% reduction in 1,25-(OH)2D3-induced 25-hydroxyvitamin-D3-24-hydroxylase activity. There was no alteration in the capacity or avidity of New World primate serum for 1,25-(OH)2D3 compared to that of serum from Old World primates. These data suggest that the occurrence of vitamin D-resistant osteomalacia in New World primates is the result of decreased high affinity, receptor-mediated uptake of 1,25-(OH)2D3 by the target cell.
我们对洛杉矶动物园皇柽柳猴新大陆灵长类动物群体中1,25 - 二羟基维生素D3[1,25-(OH)2D3]抵抗性骨软化症的发生情况进行了调查。与旧大陆灵长类动物相比，新大陆灵长类动物血清中1,25-(OH)2D3的平均浓度升高了5倍。与来自旧大陆灵长类动物或人类的细胞相比，新大陆灵长类动物培养的真皮成纤维细胞对0.6 nM[3H]1,25-(OH)2D3的特异性内化减少了75%。激素摄取的减少是由于1,25-(OH)2D3高亲和力细胞内结合位点数量的减少，这显然导致1,25-(OH)2D3诱导的25 - 羟基维生素D3 - 24 - 羟化酶活性降低了90 - 95%。与旧大陆灵长类动物血清相比，新大陆灵长类动物血清对1,25-(OH)2D3的结合能力或亲和力没有改变。这些数据表明，新大陆灵长类动物中维生素D抵抗性骨软化症的发生是靶细胞对1,25-(OH)2D3的高亲和力、受体介导的摄取减少的结果。
