蚯蚓体腔液的蛋白质分析。III. 从安德爱胜蚓中分离和鉴定几种抑菌分子。

Protein analysis of earthworm coelomic fluid. III. Isolation and characterization of several bacteriostatic molecules from Eisenia fetida andrei.

作者信息

Vaillier J, Cadoret M A, Roch P, Valembois P

出版信息

Dev Comp Immunol. 1985 Winter;9(1):11-20. doi: 10.1016/0145-305x(85)90055-2.

Abstract

A bacteriostatic activity in Eisenia fetida andrei cell free coelomic fluid is described. This activity is detected by growth inhibition of a bacteria Bacillus megaterium. Gel filtration analysis revealed eleven coelomic fluid protein fractions designated A, B,..J. Antibacterial activity was mainly found within fractions B and C. Chromatofocusing resolved fractions B-C into five different peaks named alpha BC, beta BC,... epsilon BC. Antibacterial activity appeared mediated by three different proteins characterized by their molecular weights (20,000, 40,000 and 45,000) and their isoelectric points (4.9, 5.75 and 6.0). These bacteriostatic proteins possess either hemolysis or hemagglutination activities. The polymorphic aspect of this humoral antibacterial defense is discussed.

摘要

本文描述了赤子爱胜蚓无细胞体腔液中的抑菌活性。通过巨大芽孢杆菌的生长抑制来检测这种活性。凝胶过滤分析显示，体腔液中有11个蛋白质组分，分别命名为A、B、...、J。抗菌活性主要存在于组分B和C中。色谱聚焦将组分B - C分离为五个不同的峰，命名为αBC、βBC、...、εBC。抗菌活性似乎由三种不同的蛋白质介导，其特征在于分子量（20,000、40,000和45,000）和等电点（4.9、5.75和6.0）。这些抑菌蛋白具有溶血或血凝活性。本文还讨论了这种体液抗菌防御的多态性。