Moonen P, Klok G, Keirse M J
Eur J Obstet Gynecol Reprod Biol. 1985 Mar;19(3):151-8. doi: 10.1016/0028-2243(85)90149-2.
The localisation of prostaglandin endoperoxide synthase and prostacyclin synthase in pregnant human myometrium was examined by indirect immunocytofluorescent staining using monoclonal antibodies raised against these enzymes. Both enzymes were demonstrated to be present in myometrial smooth muscle cells. Staining associated with prostacyclin synthase, however, was more intense throughout the myometrium and less circumscript than that associated with prostaglandin endoperoxide synthase. The findings indicate that the uterine smooth muscle cells themselves possess the enzymes necessary for producing prostacyclin from arachidonic acid and that this capacity is not limited to the uterine macro- and microvasculature.
使用针对这些酶产生的单克隆抗体，通过间接免疫细胞荧光染色检查了前列腺素内过氧化物合酶和前列环素合酶在妊娠子宫肌层中的定位。结果表明，这两种酶均存在于子宫肌层平滑肌细胞中。然而，与前列环素合酶相关的染色在整个子宫肌层中更强烈，且比与前列腺素内过氧化物合酶相关的染色界限更不清晰。这些发现表明，子宫平滑肌细胞自身拥有从花生四烯酸产生前列环素所需的酶，并且这种能力不限于子宫的大血管和微血管。
