Bahari Gholamreza, Taheri Mohsen, Mokhtari Mojgan, Moudi Mahdiyeh, Majidpour Mahdi, Ghadimi Hossein Shahraki
Children and Adolescent Health Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran.
Genetics of Non-Communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
Turk J Obstet Gynecol. 2024 Sep 4;21(3):158-165. doi: 10.4274/tjod.galenos.2024.74419.
Recurrent spontaneous abortion (RSA) is defined as two or more pregnancy losses before 24 gestational weeks, accounting for 1-3% of fertile couples. A vast majority of single-nucleotide polymorphisms (SNPs) in some () genes can change the miRNA-mRNA interaction and are associated with the risk of RSA. This study was designed to better elucidate the association between miR-27a, miR-499, and miR-146a polymorphisms and RSA risk.
SNP genotyping of miR-27a (rs895819), miR-499 (rs3746444), and miR-146a (rs2910164) was performed using polymerase chain reaction (PCR)-restriction fragment length polymorphism and tetra amplification-refractory mutation system PCR in 98 patients with RSA and 105 healthy subjects.
Our results showed that the miR-499 rs3746444 and miR-27a rs895819 polymorphisms were significantly associated with RSA risk, whereas no significant differences were observed between the rs2910164 polymorphism and RSA susceptibility.
We proposed that the miR-499 rs3746444 and miR-27a rs895819 polymorphisms were correlated with RSA in our population, but the miR-146a rs2910164 variant was not associated with the risk of RSA.
复发性自然流产（RSA）定义为妊娠24周前发生两次或更多次妊娠丢失，占育龄夫妇的1%-3%。某些基因中的绝大多数单核苷酸多态性（SNP）可改变miRNA与mRNA的相互作用，并与RSA风险相关。本研究旨在更好地阐明miR-27a、miR-499和miR-146a多态性与RSA风险之间的关联。
采用聚合酶链反应（PCR）-限制性片段长度多态性和四重扩增-难扩增突变系统PCR对98例RSA患者和105例健康受试者进行miR-27a（rs895819）、miR-499（rs3746444）和miR-146a（rs2910164）的SNP基因分型。
我们的结果显示，miR-499 rs3746444和miR-27a rs895819多态性与RSA风险显著相关，而rs2910164多态性与RSA易感性之间未观察到显著差异。
我们提出，在我们的研究人群中，miR-499 rs3746444和miR-27a rs895819多态性与RSA相关，但miR-146a rs2910164变异与RSA风险无关。
