Matsumoto A M, Bremner W J
J Androl. 1985 May-Jun;6(3):137-43. doi: 10.1002/j.1939-4640.1985.tb00829.x.
The precise hormonal milieu required for quantitatively normal spermatogenesis in man is unclear. The authors previously have shown that both supraphysiologic dosages of human chorionic gonadotropin (hCG) and physiologic dosages of human luteinizing hormone (hLH) can reinitiate sperm production in short-term (four months) gonadotropin-suppressed normal men who have prepubertal FSH levels. To determine whether normal FSH levels were necessary to stimulate sperm production after a prolonged period of gonadotropin and testicular suppression, the authors administered hCG to four normal men whose endogenous gonadotropin levels and sperm production were suppressed by prolonged exogenous testosterone (T) administration. After a 3-month control period, all subjects received 200 mg of T enanthate intramuscularly (im) each week to suppress LH and FSH for a total of 9 months and until successive sperm concentrations (performed twice monthly) revealed azoospermia or severe oligozoospermia (mean sperm concentration less than 3 X 10(6) spermatozoa/ml) for 6 months. Then, while continuing the same dosage of T enanthate, all four men simultaneously received 5000 IU of hCG im three times weekly for 6 months, replacing LH-like activity and leaving FSH activity suppressed. The effect on sperm production of the selective FSH deficiency produced by hCG plus T administration after the period of prolonged gonadotropin suppression was determined. Exogenous T administration resulted in severe suppression of sperm concentrations from 79 +/- 7 X 10(6) spermatozoa/ml (mean +/- SEM) during the control period to 0.8 +/- 0.5 X 10(6)/ml after 12 weeks of T treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
人类精子发生数量正常所需的精确激素环境尚不清楚。作者此前已表明，超生理剂量的人绒毛膜促性腺激素（hCG）和生理剂量的人促黄体生成素（hLH）均可使促性腺激素抑制的青春期前FSH水平的短期（四个月）正常男性重新开始产生精子。为了确定在长期促性腺激素和睾丸抑制后刺激精子产生是否需要正常FSH水平，作者对四名正常男性给予hCG，这些男性的内源性促性腺激素水平和精子产生因长期外源性睾酮（T）给药而受到抑制。在3个月的对照期后，所有受试者每周肌肉注射（im）200mg庚酸睾酮，共9个月，直至连续的精子浓度（每月进行两次）显示无精子症或严重少精子症（平均精子浓度低于3×10⁶精子/ml）持续6个月。然后，在继续使用相同剂量庚酸睾酮的同时，所有四名男性同时每周三次肌肉注射5000IU hCG，持续6个月，替代LH样活性，使FSH活性受到抑制。确定了在长期促性腺激素抑制期后，hCG加T给药所产生的选择性FSH缺乏对精子产生的影响。外源性T给药导致精子浓度从对照期的79±7×10⁶精子/ml（平均值±标准误）严重抑制至T治疗12周后的0.8±0.5×10⁶/ml。（摘要截断于250字）
