Wang Jie, Liu Jian-Ju, Tang Zhan-Ying, Liang Qian-Qian, Cui Jia-Wen
School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Spine Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Heliyon. 2024 Aug 6;10(16):e35702. doi: 10.1016/j.heliyon.2024.e35702. eCollection 2024 Aug 30.
To investigate the therapeutic effect of acupuncture on acute inflammatory nerve root injury by regulating lymphatic function.
A mouse model of L5 nerve root compression was used to simulate acute nerve root injury. After modeling, acupuncture treatment was given each day for one week. Pain thresholds were assessed before and after modeling and treatment. Immunofluorescence staining was performed to observe the distribution astrocytes and neurons in the lumbar spinal cord, the innervation rate of neuromuscular junctions (NMJs), lymphatic endothelial cells (LECs) of lumbar aortic lymph nodes, and the percentage of M1 macrophages. The number of each type of immune cells in the lumbar aortic lymph nodes (LALNs) was measured by flow cytometry.
The model group showed a significant decrease in pain threshold in the affected lower limb, while acupuncture treatment was able to significantly increase it. Acupuncture significantly repaired astrocytes and neurons in the lumbar spinal cord of the compressed segment, increased the innervation rate of nerve endings at NMJs, reduced LECs in the LALNs, reduced the proportion of M1 macrophages in the LALNs, and significantly reduced mononuclear neutrophils and monocytic neutrophils.
Acupuncture can reduce pain, promote nerve repair in mice with acute nerve root injury, and suppress immune responses in lumbar aortic lymph nodes.
通过调节淋巴功能研究针刺对急性炎性神经根损伤的治疗作用。
采用L5神经根压迫小鼠模型模拟急性神经根损伤。造模后，每天进行针刺治疗，持续一周。在造模和治疗前后评估疼痛阈值。进行免疫荧光染色以观察腰段脊髓中星形胶质细胞和神经元的分布、神经肌肉接头（NMJ）的神经支配率、腰主动脉淋巴结的淋巴管内皮细胞（LEC）以及M1巨噬细胞的百分比。通过流式细胞术测量腰主动脉淋巴结（LALN）中每种免疫细胞的数量。
模型组患侧下肢疼痛阈值显著降低，而针刺治疗能够使其显著升高。针刺显著修复了受压节段腰段脊髓中的星形胶质细胞和神经元，提高了神经肌肉接头处神经末梢的神经支配率，减少了腰主动脉淋巴结中的淋巴管内皮细胞，降低了腰主动脉淋巴结中M1巨噬细胞的比例，并显著减少了单核中性粒细胞和单核嗜中性粒细胞。
针刺可减轻疼痛，促进急性神经根损伤小鼠的神经修复，并抑制腰主动脉淋巴结中的免疫反应。
