Gao Haoli, Yuan Xiaowei, Wang Jingting, Yan Yangyang, Zhang Xinyu, He Tianshun, Lin Xumin, Zhang Huihui, Liu Zewen
Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
The Sanya Institute of the Nanjing Agricultural University, Sanya, China.
Pest Manag Sci. 2025 Jan;81(1):36-43. doi: 10.1002/ps.8403. Epub 2024 Sep 4.
The endocycle can generate cells referred to as 'polyploid'. Fizzy-related protein (Fzr) plays an important role in driving the mitosis-to-endocycle transition. The brown planthopper (BPH), Nilaparvata lugens (Stål), a serious insect pest, feeds exclusively on rice. However, polyploidy and its regulatory mechanisms are poorly understood in BPH.
Here, we found that the ploidy levels of follicles H (FH) and accessory gland (AG) significantly increased with BPH age when examining the polyploidy of FH and AG of salivary glands. Fzr was identified as an important regulator for polyploidy in BPH salivary gland. Knockdown of Fzr resulted in a decrease in cell size and DNA content in nymph salivary glands. Fzr knockdown transcriptionally upregulated cyclin-dependent kinase 1 (CDK1), CDK2, cyclin A (CycA) and CycB, and downregulated CycD, CycE, Myc and mini-chromosome maintenance protein 2-7 (MCM2-7). Phenotypically, Fzr knockdown significantly suppressed salivary protein production, feeding and survival in BPH nymphs.
Our results show that BPH salivary glands exhibit obvious polyploidy, and Fzr positively regulates the endocycle in nymph salivary gland. These findings provide clues for the study of the regulatory mechanisms of insect polyploidy. © 2024 Society of Chemical Industry.
内循环可产生被称为“多倍体”的细胞。类Fizzy蛋白（Fzr）在驱动有丝分裂向内循环转变中起重要作用。褐飞虱（BPH），即褐飞虱（Nilaparvata lugens (Stål)），是一种严重的害虫，仅以水稻为食。然而，褐飞虱的多倍体及其调控机制尚不清楚。
在这里，我们在检测唾液腺卵泡H（FH）和副腺（AG）的多倍体时发现，随着褐飞虱年龄的增长，FH和AG的倍性水平显著增加。Fzr被确定为褐飞虱唾液腺多倍体的重要调节因子。敲低Fzr导致若虫唾液腺细胞大小和DNA含量降低。Fzr敲低转录上调细胞周期蛋白依赖性激酶1（CDK1）、CDK2、细胞周期蛋白A（CycA）和CycB，并下调CycD、CycE、Myc和微小染色体维持蛋白2 - 7（MCM2 - 7）。在表型上，敲低Fzr显著抑制了褐飞虱若虫的唾液蛋白产生、取食和存活。
我们的结果表明，褐飞虱唾液腺表现出明显的多倍体，且Fzr正向调节若虫唾液腺的内循环。这些发现为昆虫多倍体调控机制的研究提供了线索。© 2024化学工业协会。
