Ferré J, Real M D, Ménsua J L, Jacobson K B
J Biol Chem. 1985 Jun 25;260(12):7509-14.
An unknown fluorescent metabolite has been isolated from heads of eye-color mutants of Drosophila melanogaster. Only a few mutations cause it to accumulate, viz. cardinal (cd), dark red brown (drb), Henna-recessive (Hnr), purple (pr), Punch2 (Pu2), Punch-Grape (PuGr), and scarlet (st). After purification by ion-exchange chromatography, the spectroscopic, chemical, and enzymatic analyses revealed that it is a novel quinoline derivative: xanthurenic acid 8-O-beta-D-glucoside. Feeding experiments suggest that this glucoside is synthesized from 3-hydroxykynurenine and that free xanthurenic acid is not a precursor. The results from the analysis for its occurrence in double mutants, together with the fact that xanthurenic acid 8-glucoside share the same precursor as xanthurenic acid and xanthommatin, suggest that xanthurenic acid 8-glucoside formation is closely related to the regulation of the last step in the biosynthesis of xanthommatin.
从黑腹果蝇眼色突变体的头部分离出了一种未知的荧光代谢物。只有少数几种突变会导致其积累，即：卡迪纳尔（cd）、深红色棕色（drb）、海娜隐性（Hnr）、紫色（pr）、庞奇2（Pu2）、庞奇-葡萄色（PuGr）和猩红色（st）。经离子交换色谱法纯化后，光谱、化学和酶学分析表明它是一种新型喹啉衍生物：8-O-β-D-葡萄糖苷黄尿酸。喂食实验表明，这种葡萄糖苷由3-羟基犬尿氨酸合成，游离黄尿酸不是前体。对其在双突变体中出现情况的分析结果，以及8-葡萄糖苷黄尿酸与黄尿酸和眼黄素共享相同前体这一事实，表明8-葡萄糖苷黄尿酸的形成与眼黄素生物合成最后一步的调控密切相关。
