Miyagi University, School of Nursing, 1-1 Gakuen, Taiwa-cho, Kurokawa-gun, Miyagi, Japan.
Miyagi University, School of Nursing, 1-1 Gakuen, Taiwa-cho, Kurokawa-gun, Miyagi, Japan,
Cell Physiol Biochem. 2024 Aug 31;58(4):445-457. doi: 10.33594/000000723.
BACKGROUND/AIMS: Lemons ( ) contain various nutrients and are among the most popular citrus fruit. Besides their antioxidant, anticancer, antibacterial, and anti-inflammatory properties, clinical studies have indicated their anti-allergic properties.
Using the differential-interference contrast (DIC) microscopy, we examined the effects of lemon juice and peel constituents, such as citric acid, ascorbic acid, hesperetin and eriodictyol, on the degranulation from rat peritoneal mast cells. Using fluorescence imaging with a water-soluble dye, Lucifer Yellow, we also examined their effects on the deformation of the plasma membrane.
Lemon juice dose-dependently decreased the number of degranulated mast cells. At concentrations equal to or higher than 0.25 mM, citric acid, hesperetin, and eriodictyol significantly reduced the number of degranulating mast cells in a dose-dependent manner, while ascorbic acid required much higher doses to exert significant effects. At 1 mM, citric acid, hesperetin, and eriodictyol almost completely inhibited exocytosis and washed out the Lucifer Yellow trapped on the mast cell surface, while ascorbic acid did not.
This study provides evidence for the first time that lemon constituents, such as citric acid, hesperetin, and eriodictyol, potently exert mast cell-stabilizing properties. These properties are attributable to their inhibitory effects on plasma membrane deformation in degranulating mast cells.
背景/目的：柠檬（ ）含有各种营养成分，是最受欢迎的柑橘类水果之一。除了具有抗氧化、抗癌、抗菌和抗炎特性外，临床研究还表明它们具有抗过敏特性。
我们使用微分干涉对比（DIC）显微镜检查了柠檬汁和果皮成分（如柠檬酸、抗坏血酸、橙皮苷和橘皮苷）对大鼠腹腔肥大细胞脱颗粒的影响。使用水溶性染料 Lucifer Yellow 进行荧光成像，我们还检查了它们对质膜变形的影响。
柠檬汁剂量依赖性地减少脱颗粒肥大细胞的数量。在浓度等于或高于 0.25 mM 时，柠檬酸、橙皮苷和橘皮苷显著地以剂量依赖性方式减少脱颗粒肥大细胞的数量，而抗坏血酸则需要更高的剂量才能发挥显著作用。在 1 mM 时，柠檬酸、橙皮苷和橘皮苷几乎完全抑制胞吐作用，并冲洗掉被困在肥大细胞表面的 Lucifer Yellow，而抗坏血酸则没有。
这项研究首次提供了证据，证明柠檬成分（如柠檬酸、橙皮苷和橘皮苷）具有强大的稳定肥大细胞的特性。这些特性归因于它们对脱颗粒肥大细胞中质膜变形的抑制作用。
