State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Key Laboratory of Integrated Pest Management on Crops in Northeast, Ministry of Agriculture, Jilin Academy of Agricultural Sciences, Changchun 130033, China.
Viruses. 2022 Feb 8;14(2):341. doi: 10.3390/v14020341.
Soybean is a major legume crop that plays an important role in food production, industrial production, and animal husbandry. Here, we characterize a novel soybean-infecting monopartite geminivirus identified in China. Analysis of the contigs assembled from sequenced small interfering RNAs, followed by PCR, cloning, and sequencing, the complete viral genome was determined to be 2782 nucleotides. The genome contains the conserved nonanucleotide sequence, TAATATTAC and other sequence features typical of the family , and encodes two and four open reading frames in the virion-sense and the complementary-sense strands, respectively. Genome-wide pairwise identity analysis revealed that the novel virus shares less than 65.6% identity with previously characterized geminiviruses. Phylogenetic and recombination analysis indicated that this virus was placed in a unique taxon within the family and potentially arose from recombination. An infectious clone of this virus was further constructed and its infectivity was tested in different species of plants. Successful infection and characteristic symptoms were observed in , , , , and cv. Samsun plants. Taken together, this virus represents a member of an unclassified genus of the family , for which the name soybean yellow leaf curl virus is proposed.
大豆是一种重要的豆科作物，在食品生产、工业生产和畜牧业中发挥着重要作用。在这里，我们描述了一种在中国发现的新型大豆侵染单分体双生病毒。通过对测序小干扰 RNA 组装的序列进行分析，随后进行 PCR、克隆和测序，确定了完整的病毒基因组为 2782 个核苷酸。该基因组包含保守的九聚体序列 TAATATTAC 和其他家族典型的序列特征，并在病毒 sense 和互补 sense 链上分别编码两个和四个开放阅读框。全基因组成对同一性分析表明，新型病毒与先前表征的双生病毒的同一性小于 65.6%。系统发育和重组分析表明，该病毒在家族内处于独特的分类群中，可能来自重组。进一步构建了该病毒的感染性克隆，并在不同植物物种中测试了其感染性。在 、 、 、 和 cv. Samsun 植物中观察到成功感染和特征性症状。综上所述，该病毒代表了家族中未分类属的一个成员，建议将其命名为大豆黄叶卷曲病毒。
