New York State Police Forensic Investigation Center, Toxicology, 1220 Washington Ave, Bldg 30, Albany, NY 12226, United States.
J Anal Toxicol. 2024 Oct 28;48(8):528-534. doi: 10.1093/jat/bkae068.
A simple and rapid qualitative chromatographic method with a unique extraction approach was developed and validated to screen oral fluid samples for 31 compounds in driving under the influence of drugs investigations. The scope and sensitivity of the method meets or exceeds Tier I recommendations established by the National Safety Council's Alcohol, Drugs and Impairment Division. Since this is a targeted chromatographic screen (rather than an immunoassay), cutoffs were set to match the confirmation levels in the recommendations. Sample preparation involved a single-step liquid-liquid extraction procedure, using a mixture of methyl tert-butyl ether, isopropanol, and hexane and was applied to samples collected with the Quantisal™ device. Instrument analysis was conducted by liquid chromatography-tandem mass spectrometry, using a Restek Raptor™ biphenyl column for chromatographic separations and a total run time of 8 min. Validation results met all requirements of ANSI/ASB Standard 036 (1st edition)-Standard Practices for Method Validation in Forensic Toxicology.
建立并验证了一种简单、快速的定性色谱方法，该方法采用独特的提取方法，用于筛选驾驶中受药物影响的口腔液样本中的 31 种化合物。该方法的范围和灵敏度符合或超过国家安全委员会酒精、药物和损伤部一级建议的要求。由于这是一种有针对性的色谱筛选（而不是免疫测定），因此设定了截止值以匹配建议中的确认水平。样品制备包括使用甲基叔丁基醚、异丙醇和正己烷的混合物进行一步液-液萃取程序，并应用于 Quantisal™装置收集的样品。仪器分析采用液相色谱-串联质谱法，使用 Restek Raptor™联苯柱进行色谱分离，总运行时间为 8 分钟。验证结果符合法医毒理学方法验证 ANSI/ASB 标准 036（第 1 版）-标准实践的所有要求。
