R&D Department Eureka Lab Division, Chiaravalle, Italy.
Department of Neuroscience, Imaging and Clinical Sciences, University of Chieti-Pescara "G. d'Annunzio", 66100 Chieti, Italy.
Forensic Sci Int. 2020 Jul;312:110330. doi: 10.1016/j.forsciint.2020.110330. Epub 2020 May 12.
A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmation method for the direct analysis of 17 drugs starting from 200μL of diluted oral fluid (OF), in a single chromatographic run, was developed and validated. Cocaine, benzoylecgonine (BEG), cocaethylene, Δ-9-tetrahydrocannabinol (Δ-9-THC), buprenorphine, 6-acetylmorphine (6AM), morphine, codeine, methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), methylenedioxyamphetamine (MDA), 3,4-Methylenedioxy-N-ethylamphetamine (MDE), ketamine, N-methyl-1-(1,3-benzodioxol-5-yl)-2-butanamine (MBDB) were determined in a chromatographic run of 12min only with no sample pre-treatment, after the addition of 15 different internal standards (ISs). The method met all requirements in terms of linearity, accuracy (precision and trueness), recovery, and stability requested by FDA guidelines. Carry-over and interferences were negligible, as well as the matrix effects. LLOQs are below the limits defined by European guidelines and Italian national laws. The original oral fluid collections are stable at least six months at -20°C and one week at +4°C.
建立并验证了一种新的液相色谱-串联质谱（LC-MS/MS）确认方法，可直接从 200μL 稀释的口服液（OF）中分析 17 种药物，单次色谱运行即可完成。可卡因、苯甲酰爱康宁（BEG）、古柯烯、Δ-9-四氢大麻酚（Δ-9-THC）、丁丙诺啡、6-乙酰吗啡（6AM）、吗啡、可待因、美沙酮、2-亚乙基-1,5-二甲基-3,3-二苯基吡咯烷（EDDP）、苯丙胺、甲基苯丙胺、3,4-亚甲基二氧基甲基苯丙胺（MDMA）、亚甲基二氧基苯丙胺（MDA）、3,4-亚甲基二氧基-N-乙基苯丙胺（MDE）、氯胺酮、N-甲基-1-（1,3-苯并二恶唑-5-基）-2-丁胺（MBDB），无需样品预处理，只需 12min 即可完成色谱运行，并添加了 15 种不同的内标物（ISs）。该方法在 FDA 指南要求的线性、准确性（精密度和准确度）、回收率和稳定性方面均符合要求。无交叉污染和干扰，基质效应也可忽略不计。LLOQs 低于欧洲指南和意大利国家法律规定的限值。原始口服液在-20°C 下至少稳定六个月，在+4°C 下稳定一周。
