Dugas B, Vazquez A, Geŕard J P, Richard Y, Auffredou M T, Delfraissy J F, Fradelizi D, Galanaud P
J Immunol. 1985 Jul;135(1):333-8.
The supernatants from PHA-activated normal human T cells (conditioned medium) were fractionated by differential adsorption on Con A-Sepharose columns. The effluent contained both IL 2 activity (tested on the CTL-L2 cell line) and BCGF activity (tested on anti-mu-activated normal B-enriched cells). Absorption of such effluent on PHA-activated T cell blasts removed the IL 2 activity without affecting the BCGF activity. The eluate also displayed BCGF activity (tested on anti-mu-activated B-enriched cells) without detectable IL 2 activity. The two BCGF species isolated by this criteria synergize with semipurified IL 1 to support the anti-mu-induced proliferation of highly monocyte-depleted B cells. Both BCGF species required the presence of anti-mu Ab to support the proliferation of small B cells (at the G0 stage). However, in contrast to crude conditioned medium and to the BCGF present in the effluent, the BCGF present in the eluate was unable to induce the proliferation of unfractionated or large (at the G1 stage) B cells in the absence of anti-mu Ab. These results support the existence of two functionally different human BCGF species, differing at least in their relative sugar content and distinct from IL 2.
用刀豆蛋白A-琼脂糖柱对PHA激活的正常人T细胞的上清液（条件培养基）进行差异吸附分级分离。流出液中同时含有IL-2活性（在CTL-L2细胞系上检测）和BCGF活性（在抗μ激活的富含正常B细胞的细胞上检测）。将这种流出液吸附到PHA激活的T细胞母细胞上可去除IL-2活性，而不影响BCGF活性。洗脱液也显示出BCGF活性（在抗μ激活的富含B细胞的细胞上检测），但未检测到IL-2活性。通过该标准分离的两种BCGF与半纯化的IL-1协同作用，以支持高度单核细胞耗竭的B细胞的抗μ诱导增殖。两种BCGF都需要存在抗μ抗体来支持小B细胞（处于G0期）的增殖。然而，与粗条件培养基和流出液中存在的BCGF不同，洗脱液中存在的BCGF在没有抗μ抗体的情况下不能诱导未分级或大的（处于G1期）B细胞增殖。这些结果支持存在两种功能不同的人BCGF，至少在其相对糖含量上有所不同，且与IL-2不同。
