Saiki I, Fidler I J
J Immunol. 1985 Jul;135(1):684-8.
Mouse peritoneal macrophages were activated to become cytotoxic against B16-BL6 melanoma cells by the combination of subthreshold amounts of murine interferon-gamma (IFN-gamma; 0.1 to 10 U/ml) and N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP; 0.001 to 10 micrograms/ml), but not by the combination of pH 2-treated IFN-gamma and MDP, heat-treated IFN-gamma and MDP, or IFN-gamma and the inactive stereoisomer of MDP, N-acetyl-muramyl-D-alanyl-D-isoglutamine (MDP-D). The encapsulation of intact IFN-gamma and MDP within the same liposome preparation was synergistic for macrophage activation. In contrast, the presentation of identical concentrations of IFN-gamma and MDP in separate liposome preparations did not activate macrophages. These data allow us to conclude that the encapsulation of genetically engineered IFN-gamma and synthetically produced MDP within the same liposome is highly efficient in producing synergistic activation of tumoricidal properties in mouse macrophages.
亚阈值量的小鼠γ干扰素（IFN-γ；0.1至10 U/ml）与N-乙酰胞壁酰-L-丙氨酰-D-异谷氨酰胺（MDP；0.001至10微克/毫升）联合使用，可激活小鼠腹腔巨噬细胞，使其对B16-BL6黑色素瘤细胞具有细胞毒性，但经pH 2处理的IFN-γ与MDP联合、热处理的IFN-γ与MDP联合，或IFN-γ与MDP的无活性立体异构体N-乙酰胞壁酰-D-丙氨酰-D-异谷氨酰胺（MDP-D）联合使用则不能激活。将完整的IFN-γ和MDP包裹于同一脂质体制剂中，对巨噬细胞激活具有协同作用。相比之下，在单独的脂质体制剂中呈现相同浓度的IFN-γ和MDP并不能激活巨噬细胞。这些数据使我们得出结论，将基因工程IFN-γ和合成产生的MDP包裹于同一脂质体中，在产生小鼠巨噬细胞杀肿瘤特性的协同激活方面效率很高。
