Carlsson M, Hedin A, Inganäs M, Härfast B, Blomberg F
J Immunol Methods. 1985 May 10;79(1):89-98. doi: 10.1016/0022-1759(85)90395-3.
A method for production of purified and concentrated mouse monoclonal antibodies was developed. The rationale for the procedure is, firstly, to expand hybridoma cell number in culture medium-containing serum; secondly, to transfer the cells to serum-free medium for production of antibodies; and finally, to harvest antibodies from the conditioned medium by means of cation exchange chromatography on SP-Sephadex C-50, followed by gel filtration on Superose 6B. When compared with chromatography of monoclonal antibodies on protein A-Sepharose our results suggest that the method is particularly useful for purification of antibodies of the IgG1 subclass. Experience from production and purification of 15 various monoclonal antibodies is reported.
开发了一种生产纯化浓缩小鼠单克隆抗体的方法。该程序的基本原理是：首先，在含血清的培养基中扩增杂交瘤细胞数量；其次，将细胞转移至无血清培养基中生产抗体；最后，通过在SP-葡聚糖凝胶C-50上进行阳离子交换色谱，然后在Superose 6B上进行凝胶过滤，从条件培养基中收获抗体。与在蛋白A-琼脂糖上进行单克隆抗体色谱分析相比，我们的结果表明该方法对IgG1亚类抗体的纯化特别有用。报告了生产和纯化15种不同单克隆抗体的经验。
