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Immobilization of proteins (antibodies) to supports with a constantly high retention of immunoreactivity involving the formation of a Schiff-base and subsequent reduction to a secondary amine with sodium borohydride or sodium cyanoborohydride has been problematical.

作者信息

Grandics P

出版信息

Appl Biochem Biotechnol. 1987 Aug;15(2):163-7. doi: 10.1007/BF02801317.

DOI:10.1007/BF02801317
PMID:3454126
Abstract
摘要

相似文献

1
Immobilization of proteins (antibodies) to supports with a constantly high retention of immunoreactivity involving the formation of a Schiff-base and subsequent reduction to a secondary amine with sodium borohydride or sodium cyanoborohydride has been problematical.将蛋白质（抗体）固定在载体上，同时保持恒定的高免疫反应性，这涉及席夫碱的形成以及随后用硼氢化钠或氰基硼氢化钠还原为仲胺，一直存在问题。
Appl Biochem Biotechnol. 1987 Aug;15(2):163-7. doi: 10.1007/BF02801317.
2
An essential role for constitutive Schiff base-forming ligands in antigen presentation to murine T cell clones.
J Immunol. 1990 Apr 15;144(8):2883-90.
3
[The use of siliceous immunosorbents in hemosorption].[硅质免疫吸附剂在血液吸附中的应用]
Patol Fiziol Eksp Ter. 1989 Sep-Oct(5):62-5.
4
[Optimization of methods of immobilization of anti-immunoglobulins. Equilibrium parameters of the interaction of immobilized antibodies with an antigen].
Biokhimiia. 1988 Feb;53(2):238-50.
5
[Clinical immunosorbents on a basis of polymeric matrices].基于聚合物基质的临床免疫吸附剂
Bioorg Khim. 1988 Nov;14(11):1524-9.
6
Reversed immunosorbents: a simple method for specific antibody immobilization.反向免疫吸附剂：一种固定特异性抗体的简单方法。
J Immunol Methods. 1978;22(1-2):131-42. doi: 10.1016/0022-1759(78)90065-0.
7
Effect of borohydride reduction on antibodies.硼氢化物还原对抗体的影响。
Appl Biochem Biotechnol. 1987 Mar;14(2):91-9. doi: 10.1007/BF02798427.
8
Novel effective immunoadsorbents based on agarose-polyaldehyde microsphere beads: synthesis and affinity chromatography.
Anal Biochem. 1983 Feb 1;128(2):342-50. doi: 10.1016/0003-2697(83)90384-6.
9
Evidence for an intercellular covalent reaction essential in antigen-specific T cell activation.
J Immunol. 1989 Sep 1;143(5):1482-9.
10
[Preparation of different immunosorbents and their use for isolation of antigens and antibodies].
Vopr Med Khim. 1976 Jan-Feb;22(1):127-36.

本文引用的文献

1
Alternative reducing agents for reductive methylation of amino groups in proteins.用于蛋白质中氨基还原甲基化的替代还原剂。
Int J Pept Protein Res. 1981 Mar;17(3):345-52. doi: 10.1111/j.1399-3011.1981.tb02001.x.
2
A rapid solution-phase screening technique for hybridoma culture supernatants using radiolabeled antigen and a solid-phase immunoadsorbent.一种使用放射性标记抗原和固相免疫吸附剂对杂交瘤培养上清液进行快速溶液相筛选的技术。
J Immunol Methods. 1983 May 13;59(3):277-80. doi: 10.1016/0022-1759(83)90188-6.
3
Some monoclonal antibodies raised with a native protein bind preferentially to the denatured antigen.
一些针对天然蛋白质产生的单克隆抗体优先结合变性抗原。
Mol Immunol. 1984 Jul;21(7):673-7. doi: 10.1016/0161-5890(84)90053-1.
4
The direct coupling of oligosaccharides to proteins and derivatized gels.寡糖与蛋白质及衍生化凝胶的直接偶联。
Arch Biochem Biophys. 1974 Jul;163(1):426-8. doi: 10.1016/0003-9861(74)90495-0.
5
Reductive alkylation of proteins with aromatic aldehydes and sodium cyanoborohydride.蛋白质与芳香醛和氰基硼氢化钠的还原烷基化反应。
Int J Pept Protein Res. 1974;6(3):183-5. doi: 10.1111/j.1399-3011.1974.tb02377.x.
6
The influence of methods of production, purification and storage of monoclonal antibodies upon their observed specificities.
J Immunol Methods. 1985 Jun 25;80(2):189-97. doi: 10.1016/0022-1759(85)90020-1.
7
Purification of in vitro produced mouse monoclonal antibodies. A two-step procedure utilizing cation exchange chromatography and gel filtration.体外产生的小鼠单克隆抗体的纯化。采用阳离子交换色谱法和凝胶过滤法的两步法。
J Immunol Methods. 1985 May 10;79(1):89-98. doi: 10.1016/0022-1759(85)90395-3.
8
Effect of borohydride reduction on antibodies.硼氢化物还原对抗体的影响。
Appl Biochem Biotechnol. 1987 Mar;14(2):91-9. doi: 10.1007/BF02798427.
9
Factors affecting the properties of insolubilized anitbodies.影响固定化抗体性质的因素。
Biochim Biophys Acta. 1976 Feb 20;420(2):246-57. doi: 10.1016/0005-2795(76)90316-0.
10
Labeling of proteins by reductive methylation using sodium cyanoborohydride.使用氰基硼氢化钠通过还原甲基化对蛋白质进行标记。
J Biol Chem. 1979 Jun 10;254(11):4359-65.