Research Institute of Plant Protection, Guangdong Academy of Agricultural Sciences & Key Laboratory of Green Prevention and Control of Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs & Key Laboratory of High Technology for Plant Protection of Guangdong Province, Guangzhou 510640, China.
Institute of Agricultural Facilities and Equipment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
J Agric Food Chem. 2024 Sep 18;72(37):20646-20657. doi: 10.1021/acs.jafc.4c06316. Epub 2024 Sep 4.
Plants have complex detoxification and metabolic systems that enable them to deal with environmental pollutants. We report accumulation of the pesticide isoproturon (IPU) in a BR signaling pathway for mutant 3/5 rice to be significantly higher than in wild-type (WT) rice controls and for exogenous 24-epibrassinolide to reverse toxic symptoms in WT rice but not in mutants. A genome-wide RNA sequencing study of WT/ rice is performed to identify transcriptomic changes and metabolic mechanisms under IPU exposure. Three differentially expressed genes in yeast cells increase the degradation rate of IPU in a growth medium by factors of 1.61, 1.51, and 1.29 after 72 h. Using UPLC/Q-TOF-MS/MS, five phase I metabolites and five phase II conjugates are characterized in rice grains, with concentrations generally decreasing in rice grains. OsBZR4, a regulator of IPU degradation in rice, may eliminate IPU from edible parts of food crops by regulating downstream metabolic genes.
植物具有复杂的解毒和代谢系统，使它们能够应对环境污染物。我们报告说，在 BR 信号通路中，突变体 3/5 水稻对杀虫剂异丙隆（IPU）的积累明显高于野生型（WT）水稻对照，而外源 24-表油菜素内酯则能逆转 WT 水稻的毒性症状，但不能逆转突变体的毒性症状。对 WT/水稻进行全基因组 RNA 测序研究，以确定在 IPU 暴露下的转录组变化和代谢机制。在酵母细胞中，三个差异表达基因在生长培养基中使 IPU 的降解率在 72 小时后分别增加了 1.61、1.51 和 1.29 倍。使用 UPLC/Q-TOF-MS/MS，在水稻籽粒中鉴定出 5 种 I 期代谢物和 5 种 II 期轭合物，其浓度通常在 3/5 水稻籽粒中降低。OsBZR4 是水稻中 IPU 降解的调节剂，可能通过调节下游代谢基因来消除食用部分的农作物中的 IPU。
